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《Journal of Anhui Agricultural Sciences》 2007-36
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Study on the Property of Producing Cellulase by a Strain of Aspergillus niger

FENG Pei-yong et al(College of Life Science,Ludong University,Yantai,Shandong 264025)  
[Objective] The aim of the study was to provide foundation for the practical application of Aspergillus niger.[Method] The crude enzyme solution of cellulase was obtained by shake-flask culture of A.niger and the activities of Cl,Cx and BG enzymes were determined to study the influences of temperature,pH value,substrate concentration and metal ions on the activities of different enzyme components.[Result] The cellulase excreted by A.niger was more complete,but the exudation peaks of different enzyme components were disaccording.The optimum temperature of Cl and BG enzymes was 60 ℃,and that of Cx enzyme was 70 ℃.The optimum pH value of Cx and BG enzymes was 4~5,that of Cl enzyme was about 4.The activities of Cl and BG enzymes increased along with the increment of substrate concentration,and was highest when the substrate concentration was 1.000%.The activity of Cx enzyme was highest when the substrate concentration was 0.500%~0.625%.Ca2+ and Fe2+ had strong restraining effect on the enzymes,Ba2+ and Mn2+ had some activation effect on the enzymes,and Zn2+ and Cu2+ had restraining effect on the activity of Cx and Cl enzymes and activation effect on BG enzyme.[Conclusion] The influences of different reaction conditions on the activities of different enzyme components were different.The cellulase system of A.niger was most stable when the temperature was 60~70 ℃ and pH values were 4~5.
【Fund】: 鲁东大学校基金(20053305);; 鲁东大学大学生科技创新基金(06B077)
【CateGory Index】: Q93
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