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《Journal of Anhui Agricultural Sciences》 2007-36
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Study on Optimization for RAPD Reaction System and Genetic Relationship in Punica granatum

LIU Su-xia et al(Henan Normal University,Xinxiang,Henan 453007)  
In this study,orthogonal design was used to optimize RAPD amplification system on pomegranate of four factors:Mg2+,dNTP,primers and Taq DNA polymerase in three levels respectively.A suitable RAPD reaction system was established,namely,25 μl reaction system containing 1×PCR buffer,2.5 mmol/L MgCl2,200 μmol/L dNTP,0.2 μmol/L primer,50 ng DNA template and 0.5 U Taq DNA polymerase.The optimal annealing temperature for RAPD-PCR reaction system was proposed by gradient PCR.On the basis of this optimal reaction system,the systematic classification of 11 cultivars of pomegranate was studied.It was revealed that the polymorphism of RAPD mark in the 11 cultivars was very high,and the variance was large.The result of clustering also showed that 11 cultivars could be classified into two groups:Linxuan No.1,Jingpitian,Xindatian,Linxuan No.14;Dalisanbaitian,Daqingpisuan,Xiaohongpisuan,Baihua,Jiangshiliu,Baopizao and Yushizi.So this optimal reaction adapted to the RAPD reaction.
【Fund】: 河南省自然科学基金项目(0611030300)
【CateGory Index】: S665.4
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【References】
Chinese Journal Full-text Database 4 Hits
1 FAN Qing-jun1,LV Hui1,FAN Yi-rong1,ZHENG Yan-wei2(1.Zhejiang Agricultural and Forestry University School of Landscape Architecture,Lin'an,Zhejiang 311300;2.Highway Management Part of Shangyu City,Shangyu,Zhejiang 312300);Optimization of the RAPD-PCR Reaction System of Peaonia suffruticosa by Orthogonal Design[J];Northern Horticulture;2011-05
2 WANG Chao1,LI Rongqin1,GUAN Fachun1,WANG Yanjie2,LI Jing3,XU Jing3,TAO Lei3(1.Tibet Agriultural and Animal Husbandry College,Lizhi Tibet 860000,China;2.Chengdu Institute of Biology,Chinese Academy of Science,Chendu 600041,China;3.College of Life Sciences,Northeast Forestry University,Harbin 150040,China);Optimization of RAPD reaction system of Fragaria ananassa Duch.[J];Journal of Northeast Agricultural University;2011-04
3 LA Ping~1,LUO Shu-ping~1,ZHANG Jian-xin~1,GUO Chang-kui~1, LIU Huan-xia~2,MA Hao~3,ZHAO Tu-qiang~2 (1.College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China;2.Shihhotze Institute of Beta Vulgaris,Shihhotze 832011,China;3.Nanjing Agricultural Agricultural University, Nanjing 210095,China);Study on Optimization for RAPD Reaction System and Genetic Relationship in Beta vulgaris[J];Journal of Xinjiang Agricultural University;2010-01
4 Li Ni 1,Yan Liping 2,3,Liu Cuilan 2,3,Qu Xing 1,Liang Huimin 4,Xia Yang 2,3(1 College of Agronomy,Gansu Agricultural University,Lanzhou 730070;2 Shandong Provincial Academy of Forestry,Jinan 250014;3 Shandong Provincial Key Laboratory of Forest Genetic Improvement Tree,Jinan 250014;4 Jiangsu Agriculture and Forestry Profession Technology College,Jurong Jiangsu 212400);Optimization of RAPD System for Photinia faseri[J];Chinese Agricultural Science Bulletin;2012-34
China Proceedings of conference Full-text Database 2 Hits
1 Xue Huabo Cao Shangyin Guo Junying Liu Li Tan Honghua Zhang Sen Li Haoxian (Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences Zhengzhou 450009);Brief Review of Research on Genetic Diversity of Pomegranate Germplasm[A];[C];2010
2 Li Haoxian Cao Shangyin Guo Junying Xue Huabai Liu Li Tan Honghua Zhang Sen (Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences Zhengzhou 450009);The Types and Application of Molecular Markers in the Study of Pomegranate Germplasm Resources[A];[C];2010
【Citations】
Chinese Journal Full-text Database 10 Hits
1 XU Ying-bi~1,ZHOU Xian-feng~1,YIN Biao~1,GUO Ling~2,DING Zhien~1(1.Anhui Agricultural University,Hefei 230036,Anhui,China;2.Nongken University of Tarim in Xinjiang);Isozyme Analyses of Four Varieties of Punica granatum[J];Protection Forest Science and Technology;2006-02
2 NIE Zhen-su 1,2,3, LAI Zhong-xiong 1,2*, PAN Dong-ming2, GUO Yu-qiong 1,2, CHEN Yi-ting1, DENG Chuan-yuan 1,2, CHEN Gui-xin 2, WU Jin-shou 1,2 (1. Institute of Horticutural Biotechnology; 2. College of Horticulture; 3. Coll ege of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Genomic DNA extraction and optimization of RAPD amplifying conditions of Canar ium album Reausch[J];Sugarcane;2005-02
3 ZHANG Jian-cheng~1,QU Hong-zheng~1,ZHANG Xiao-wei~2(1 Department of Horticulture, Shanxi Agriculture University,Taigu 030801,China;2 Institute of Pomology,Shanxi Academy of Agricultural Science,Taigu 030800,China);Research advances of pomegranate in China[J];Hebei Journal of Forestry and Orchard Research;2005-03
4 He Zhengwen Liu Yunsheng Chen Lihua Cao Meihong(Department of Neurosurgery, Xiangya Hospital, Hunan Medical University, Changsha 410008)Xia Jiahui(The State Key Lab. of Medical Genetics of China, Changsha 410078);Orthogonal design-direct analysis for PCR optimization[J];BULLETIN OF HUNAN MEDICAL UNIVERSITY;1998-04
5 XU JIURU, Beijing Forestry University ZHAO BINGLUN and WANG SHENGMIN. Forestry Department of Shaanxi Province;Study on Genetic Resouces of Pomegranate[J];Economic Forest Researches;1993-01
6 WANG Xiao-fei~(1,2),XIANG Qi-bai~(1*),YOU Chuan-kai~3,WANG Yu-yi~2(1.College of Forest Resources and Environment Nanjing Forestry University,Nanjing 210037,China;2.Department of Resources and Environment Huangshan College,Huangshan 245041,China;3.The Science and Technology Development Center of Scenery of Anhui Province,Hefei 230031,China);Studies on Cultivar Classification of Punica granatum[J];Journal of Nanjing Forestry University(Natural Sciences Edition);2006-04
7 BIAN Cai miao,LI Jun min,JIN Ze xin,GE Ming ju (Biology and Chemistry Department,Taizhou Teachers College,Linhai,Zhejiang 317000,China);Effect of BSA on Random Amplified Polymorphic DNA(RAPD) in Plants[J];Hereditas(Beijing);2002-03
8 YANG Rong-ping,LI Wen-xiang,LONG Wen-hong,YANG Zheng-an(College of Landscape and Horticulture,Y A U,Kunming 650201,China);Comparison of DNA Extraction Methods and Effect of Antioxidants on Quality of DNA in Pomegranate[J];Journal of Yunnan Agricultural University;2005-05
9 Cui Chengri~ 1 ,Xu Qijiang~ 2 ,Cui Chongshi~ 1 , and Li Jingpeng~ 3* ( ~1Colllege of Horticulture,Northeast Agricultural University,Harbin,Heilongjiang 150030, China; ~2Colllege of Life Science,Northeast Forestry University,Harbin,Heilongjiang 150040, China; ~3Colllege of Life Science,Northeast Agricultural University,Harbin, Heilongjiang 150030, China);An Analysis of Onion Genetic Diversity by RAPD[J];Acta Horticulturae Sinica;2006-04
10 Wang Xiao quan, Zou Yu ping, Zhang Da ming, Zhang Zhi xian and Hong De yuan (Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Academia Sinica, Beijing 100093);PROBLEMS IN THE USE OF RAPD TO THE STUDY OF GENETIC DIVERSITY AND SYSTEMATICS[J];Acta Botanica Sinica;1996-12
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Han~(1,2),Gao Yong-chao~3,SHA Wei~2,LI Ru-yu~1,DAI Shuang~1(1.Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China;2.Department of Biology,College of Life Science and Engineering,Qiqihar University,Qiqihar 161006;3.Biotechnology Research Center of Shandong Academy of Sciences,Jinan 250014);Study of DNA extraction and genetic diversity in grimmiaceae[J];Shandong Agricultural Sciences;2006-01
2 ZHANG Qing-ning1,HU Ming2,ZHU Rong-sheng2,REN Xiang-quan3,WU Ying2,WANG Huai-zhong2,LIU Yu-qing2,WANG Shu-bai1(1.Qingdao Agricultural University,Qingdao 266109,China;2.Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan 250100,China;3.Livestock Technology Company of Dilisi Group,Zhucheng 262216,China);Microbial Properties and Application of Superior Bacteria in Deep-litter Ecosystem for Piggery[J];Shandong Agricultural Sciences;2009-04
3 ZENG Bing et al (Department of Grassland Science, Sichuan Agricultural University, Yaan, Sichuan 625014);Optimal of RAPD Marker in Dactylis glomerata[J];Journal of Anhui Agricultural Sciences;2005-07
4 PEI Guo-liang et al (College of Bio-information,Northwest Science and Technology University of Agricultural and Forestry,Yangling,Shaanxi 712100);Study on RAPD Reaction System for Gene Difference of the Cold and Warm Type of Wheat[J];Journal of Anhui Agricultural Sciences;2006-13
5 ZHAO Xin-liang et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Optimization of SRAP System in Maize[J];Journal of Anhui Agricultural Sciences;2006-15
6 LI Jian-hui et al (College of Life Science,Hangzhou Teachers College,Hangzhou,Zhejiang 310018);Establishment and Optimization of ISSR Reaction System of Schima superba[J];Journal of Anhui Agricultural Sciences;2006-19
7 ZHOU Yu-bi et al (Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,Qinhai 810001);Effect of BSA on RAPD Analysis of Cynomorium songaricum[J];Journal of Anhui Agricultural Sciences;2007-28
8 YI Dao-sheng et al(Huo Ying-dong College of Bioengineering,Shaoguan College,Shaoguan,Guangdong 512005);Multiplex PCR Detection and Typing of Porcine Circovirus[J];Journal of Anhui Agricultural Sciences;2007-32
9 XUE Zhi-zhong et al(College of Biological Science and Engineering,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018);DNA Extraction from Oil Sunflower Seeds and the Optimization of Its RAPD Reaction System[J];Journal of Anhui Agricultural Sciences;2008-06
10 HUANG Zi-feng et al(Dongguan Institute of Agricultural Seeds in Guangdong Province,Dongguan,Guangdong 523063);Optimization of RAPD Reaction System for Lilium formolongi[J];Journal of Anhui Agricultural Sciences;2008-18
China Proceedings of conference Full-text Database 10 Hits
1 Feng Lijuan Yuan Zhaohe~* Yin Yanlei Zhao Xueqing Tao Jihan (Shandong Institute of Pomology Taian 271000);Research Progress on the Domestic and Foreign Pomegranate Germplasm[A];[C];2010
2 Xue Huabo Cao Shangyin Guo Junying Liu Li Tan Honghua Zhang Sen Li Haoxian (Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences Zhengzhou 450009);Brief Review of Research on Genetic Diversity of Pomegranate Germplasm[A];[C];2010
3 Cheng Jinghui~1 Wu Xingen~1 Zhang Ying~2 Yang Rongping~1 Wu Shaobo~1,Li Wenxiang~1 (1.Landscape and Horticulture College of Yunnan Agricultural University Kunming 650201; 2.Agriculture Bureau of Mengzi County Mengzi 661100);Study on Dynamic Changes of Nutrient Material Contents in Pomegranate Fruit[A];[C];2010
4 Hu Xiaoxue~1 Wu Xingen Li Wenxiang (1.Landscape and Horticulture College of Yunnan Agricultural University Kunming 650201;2.Agriculture Bureau of Mengzi County Mengzi 661100);Study on the Dynamic Changes of Pigment Contents During Fruit Growing and Development of Pomegranate[A];[C];2010
5 Zhang Yanping~1 Cao shangyin~(2*) Chu jianqing~1 Xue huabo~2 Wang wenyan~1 Fang jinggui~1 (1.College of Horticulture,Nanjing Agricultural University Nanjing 210095; 2.Institute of Pomology in Zhengzhou,Chinese Academy of Agricultural Sciences Zhengzhou 450009);The Detection of PCR Amplified Products of Pomegranate RAPD Using Two Different Electrophoresis Systems and Sequencing Analysis of Them[A];[C];2010
6 SHI Qian-qian WANG Yan ZHOU Lin (Research Institute of Forestry,CAF,SFA Key of Breeding and Cultivation,Beijing 100091);Optimization of SRAP-PCR System and Selection of Primers for Paeonia suffruticosa Based on Capillary Electrophoresis[A];[C];2011
7 Zhang Huping~1 Niu Jianxin~1 Wang Guo'an~2 Hu Haifang~2 Ma Binggang~1 Zhu Jun~1 (~1Department of Horticulture.College of Agriculture.Shihezi University,Shihezi 832003,China;~2 Xinjiang Forestry Academy.Urumqi 830000,China);Establishment of Optimal Reaction System of RAPD in Walnut[A];[C];2003
8 Ma Yan-zhi~(1,2) Zhang Yu-xing~1 (1 College of Horticulture,Agricultural University of Hebei,Baoding Hebei 071001;2 Department of Biological Science, Tangshan Teacher's College,Tangshan Hebei 063000);Effect of Genome DNA Extracted from Different Tissues on RAPD in Pyrus L.[A];[C];2009
9 HU Yong-hong JIANG Chang-hua ZHENG Zhong-guo WANG Rong LI Jian WANG Jia WANG Li-cui ZHANG Ya-li (Research Center of Shanghai Botanical Garden,Shanghai 200231);Progress in Research of Flowering Shrubs[A];[C];2009
10 HOU Bo-xin~1 LIN Feng~1 DONG Hai-yan~2 CHEN Xiao-lin~3 (1 Hunan Forestry Academy,Changsha 410004;2 Zhejiang Forestry College,Lin'an 311300; 3 Central South University of Forestry & Technology,Changsha 410004);Classification System to Variety of Lorpetalum chinense ‘Rubrum'[A];[C];2010
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Hong et al (College of Science,Honghe University,Mengzi,Yunnan 661100);Quality Analysis of Different Cultivars of Pomegranate[J];Journal of Anhui Agricultural Sciences;2006-03
2 MIN Yong et al (The Chemistry Department of Honghe University,Mengzi,Yunnan 661100);Study on Chemical Constituents of Punica Granatum[J];Journal of Anhui Agricultural Sciences;2006-12
3 LI Rui-huan et al(College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100);Genomic DNA Extraction and RAPD System Optimization of Chinese Jujube[J];Journal of Anhui Agricultural Sciences;2007-17
4 XUE Zhi-zhong et al(College of Biological Science and Engineering,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018);DNA Extraction from Oil Sunflower Seeds and the Optimization of Its RAPD Reaction System[J];Journal of Anhui Agricultural Sciences;2008-06
5 CHEN Xia 1,2 CHEN Shao-yu 2 LU Bin 2 LI Wen-xiang 1 ZHANG Yu 2(1 Yunnan Agriculture University,Kunming Yunnan 650201;2 Yunnan Academy of Forestry);Molecular Markers and its Applications in Germplasm Research of Walnut[J];;2009-14
6 ZHANG Shui ming 1,ZHU Li wu 1,QING Ping le 2,LI Shao wen 1,WANG Dao shun 3,LOU Zhi 3 (1.Deptartment of Horticulture,Anhui Agricultural University,Hefei 230036; 2.Forestry Bureau of Dingyuan County;3.Agriculture Bureau of Huaiy;Synthetic Valuation on Economic Character of Pomegranate Cultivars in Anhui[J];Journal of Anhui Agricultural University;2002-03
7 LU Li-juan,GONG Xue-mei,ZHU Li-wu(Key Laboratory of Pomology,Anhui Agricultural University,Hefei 230036);Study on seed hardness of pomegranate cultivars in China[J];Journal of Anhui Agricultural University;2006-03
8 ZHU Hong-xia(Shanghai Polytechnic College of Urban Management,Shanghai 200438 China);DNA Template Preparation and Technology System Construction for AFLP Analysis in Paeonia suffruticosa[J];Northern Horticulture;2007-10
9 Zhang Chunxiao Li Yue Shen Xihuan (College of Forest Resources and Environment,Beijing For.Univ.,100083,P.R.China);A Review on Forest Trees Genetic Diversity at the Isozyme Level[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1998-03
10 GUO Hai lin,LIU Jian xiu,GUO Ai gui,LIU Xue shi (Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014);A Preliminary study on Chromosome Number Variation of Cynodon dactylon[J];Acta Agrestia Sinica;2002-01
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 TAN Xue,GUO Shuang,ZHENG Shao-wei,LIU Zi-zhu,LIN Jin-ying,LIN Chun-hua(Guangzhou Academy of Agricultural Sciences,Guangzhou 510308,China);Genetic Relationship Analysis of Flowering Chinese Cabbage Germplasm Resources by RAPD[J];Acta Agriculturae Boreali-Sinica;2011-S1
2 ZHAO Li-hua(College of Agricultural Sciences,Xichang College,Xichang 615000,China);Genetic diversity of pomegranate(Punica granatum L.) population in China by RAMP markers[J];Jiangsu Journal of Agricultural Sciences;2013-03
3 ZHOU Jing-hua1,JIE Yu-cheng1,2*,DU Xiao-hua,XING Hu-cheng1,2,XIONG Li-fu3(1 Institute of Ramie,Hunan Agricultural University,Changsha,Hunan 410128,China;2 Hunan Provincial Key Laboratory of Corp Germplasm Innovation and Utilization,Changsha,Hunan 410128,China;3 Changsha Arrowroot Engineering Technology Research Center,Changsha,Hunan 410005,China);RAPD Analysis of Arrowroot( Pueraria L. ) Germplasm Genetic Relationship[J];Crop Research;2013-04
China Proceedings of conference Full-text Database 1 Hits
1 Feng Lijuan Yuan Zhaohe~* Yin Yanlei Zhao Xueqing Tao Jihan (Shandong Institute of Pomology Taian 271000);Research Progress on the Domestic and Foreign Pomegranate Germplasm[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Shui ming 1,ZHU Li wu 1,QING Ping le 2,LI Shao wen 1,WANG Dao shun 3,LOU Zhi 3 (1.Deptartment of Horticulture,Anhui Agricultural University,Hefei 230036; 2.Forestry Bureau of Dingyuan County;3.Agriculture Bureau of Huaiy;Synthetic Valuation on Economic Character of Pomegranate Cultivars in Anhui[J];Journal of Anhui Agricultural University;2002-03
2 Cheng Suozhan Yang Wenheng;Some Issues on Studying the Cultivated Plant Taxonomy and Origin by Means of Isozyme Analysis[J];Journal of Agricultural University of Hebei;1986-02
3 WU Zhen,SHE Xiaoping,WANG Zhezhi(College of Life Science,Shaanxi Normal University,Xian 710062,China);Comparison on extraction methods of Ziziphus jujuba and Ziziphus acidojujuba DNA for RAPD analysis[J];Acta Botanica Boreali-occidentalia Sinica;2003-04
4 Zhao Xiangui and Xial Ling(Northweastern College of Forestry, Yangling. Shaanxi 712100);A STUDY ON POLLEN MORPHOLOGY OF PUNICACEAE FROM CHINA[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1996-01
5 WANG Feng hua 1, LAI Zhong xiong 1, CHEN Gui xin 1, LU Liu xin 1, ZHENG Jin gui 2 (1.College of Horticulture, Fujian Agriculture and Forestry University; 2.College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fuji;Advances in molecular markers and gene engineering in fruit trees[J];Journal of Fujian Agricultural University(Natural Science);2001-02
6 Feng Yuzeng;Chen Dejun;Song Meiting;Zhao Yanli;Li Zhanhong and Li Zongquan;Assessment and Utilization of the Pomegranate Variety Resources in Henan Province[J];JOURNAL OF FRUIT SCIENCE;1998-04
7 Chen Huiming 1 Zhou Changjiu 2 (1 Hunan Vegetable Research Institute,Changsha,410125) (2 Department of Horticulture,CAU,Beijing,100081);A Study on Genetic Relationship between Radishs Esterase Isoenzymes and Varieties[J];Journal of Hunan Agricultural University;1999-03
8 HU Xiong-gui,HONG Ya-hui,ZHANG Xue-wen,HUANG Li-hua (Laboratory of Cell and Gene Engineering,HNAU,Changsha410128,PRC);An Isoenzyme Analysis of Different Kinds Hemerocallis citrina[J];Journal of Hunan Agricultural University;2003-06
9 LEI Ning fei,SU Zhi xian,CHEN Jin song (Sichuan Provincial Key Laborastory of Environmental science and Biodiversity Conservation,Research Center of Biodiversity,Sichuan Teachers College,Nanchong 637002,China);Application of Isozyme Technology to the Study of Plants[J];Journal of Sichuan Teachers College(Natural Science);2000-04
10 XU JIURU, Beijing Forestry University ZHAO BINGLUN and WANG SHENGMIN. Forestry Department of Shaanxi Province;Study on Genetic Resouces of Pomegranate[J];Economic Forest Researches;1993-01
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