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《Journal of Anhui Agricultural Sciences》 2008-01
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Extraction of Phycocyanin from Nostoc commune Vanch.

LIANG Wen-yu (College of Life Sciences,Ningxia University,Yinchuan,Ningxia 750021)  
[Objective] The research aimed to provide experimental and theoretical basis for the extraction and development of phycocyanin from Nostoc commune Vanch.[Method] With N.commune as materials,the effects of crushing methods for alga and cell,the types of extraction liquid and the concentration of ammonium sulfate on the extraction of phycocyanin were compared.[Result] With 0.05 mol/L KP buffer solution(pH value of 7.2) as extraction liquid, alga and cell were crushed by fermentation method.Through salting-out with saturated ammonium sulfate and DEAE-Toyopeal 650 S ion exchange column chromatography,the purity of phycocyanin reached 2.51 and the maximum ultraviolet-visible absorption peak was at 616 nm.[Conclusion] The more ideal procedure of extracting phycocyanin from N.commune was as follows: algae powder→soaking with 0.05mol/L KP buffer solution(pH value of 7.2)→crushing cells by fermentation method→salting out with 35%~50% saturated ammonium sulfate→DEAE-Toyopeal 650 S column chromatography→gaining phycocyanin with high purity.
【Fund】: 宁夏大学科学研究基金项目(ZR0619)
【CateGory Index】: Q946
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