Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Agricultural Sciences》 2008-02
Add to Favorite Get Latest Update

Study on the Synthesis of Lactoferricin Bovine (Lfcin B) Gene and Its Expression in Microzyme

MA Feng-long et al (Shandong Xinde Pharmaceutical CO., LTD., Qingdao,Shandong 266061)  
[Objective] The research aimed to provide basis for clinical research and cure the infection caused by some pathogenic microorganisms.[Method] According to the amino acid sequence of Lfcin B, the gene was synthesized by using chemical synthesis method and cloned into pPIC9K vector. Then it was transformed into competent cells of microzyme GS115 and high-copy microzyme transformants were screened out by using resistant selective marker G418. The expression of the recombinant Lfcin B gene was induced by methanol and the expression conditions of target protein Lfcin B were detected by Tricine- SDS- PAGE and at the same time the antibacterial activity of expression product was detected by bacteriostatic test in vitro. [Result] An expected specific band with the length of about 560 bp was amplified by the extracted microzyme DNA. The target gene was successfully cloned into pPIC9K vector and transformed into microzyme GS115 for obtaining expression. The antibacterial activity of antibacterial peptide of Lfcin B in expression supernatant of the recombinant yeast to Salmonella enteritidis was 42.857 IU/ml, which indicated that yeast expression vector of Lfcin B gene was constructed successfully. [Conclusion] This research proved that it was feasible to construct eukaryotic expression vector by directly synthesizing DNA coded by Lfcin B by using chemical synthesis method and preparing Lfcin B by biological fermentation engineering.
【Fund】: 山东省博士后基金资助项目
【CateGory Index】: Q78
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 3 Hits
1 BA Te1,LIU Cheng-chu1,YANG Dan1,XIE Jing1,LI Ying-sen2,LI Jia-le2,3 (1.College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306;2.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Education,Shanghai 201306;3.Aquaculture Division,E-Institute of Shanghai Universities,Shanghai 201306);Optimization for the Fermentation Medium and Expression Conditions of the Engineered Bacteria(E. coli-pED-LfcinB BL21) Encoding Lactoferricin[J];Liaoning Agricultural Sciences;2010-05
2 Bate,Ding Zhuo-ping,Liu Cheng-chu(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China);Overview on Antimicrobial Activities of Lactoferricin and Its Genetic Engineering Production[J];Food and Fermentation Industries;2010-07
3 LI Zhong-qing1,2,3,WANG Liang1,3,(1.Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,China;2.Xinjiang Jinniu Biology Co.Ltd.,Urumqi 830026,China;3.Graduate University,Chinese Academy of Sciences,Beijing 100039,China);Current Status and Future Prospects of Bovine Lactoferricin[J];Food Science;2011-13
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 LI Gui-ping1,2,3,CHEN Yi-ben1,2(1.Guangdong Institude of Microbiology,Guangzhou 510070;2.Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangzhou 510070;3.South China Sea Institude of Oceanology,Guangzhou 510301);Antibacterial peptides and its industry application[J];Industrial Microbiology;2007-05
2 XU Jin Shu, ZHANG Shuang Quan (School of Life Science, Nanjing Normal University, Nanjing 210097, China);Advances in the research of the functions of insect antibacterial peptides against pathogenic organisms[J];Acta Entomologica Sinica;2002-05
3 YANG Xiao-hong1, ZHANG Qing-hua2, DUAN Xi-ling1, DONG Jian-zhen3,HOU Yu-ying2, ZHAO Rui-jun2 (Department of Parasitology,Shanxi Medical College of Continuing,Taiyuan 030012,China);Anticancer Activity of Antimicrobial Peptides Isolated from Three Insects[J];Journal of Tropical Medicine;2006-06
4 LIU Yue-huan1,WU Jiu-sheng2,SHI Zhang-Kui1(1.Zhejiang Academy of Medical Sciences,Hangzhou 310013,China;2.College of Animal Science,Zhejiang University,Hangzhou 310029,China);Clone and Identification of β-defensin Gene in Mongolian Gerbil[J];Laboratory Animal and Comparative Medicine;2008-04
5 Hu Yunlong Zhao Xuezhong Qu Xianming;The Study Advance in Molecular Biology of Antibacteria Peptides[J];PROGRESS IN BIOTECHNOLOGY;1997-03
6 LIU Yue-huan(Zhejiang Centre of Laboratory Animal,Zhejiang Academy of Medical Sciences,Hangzhou 310013,China);Clone and Identification of β-defensin(defβ-83) Gene in the Mongolian Gerbil[J];Chinese Journal of Comparative Medicine;2008-09
7 JIANG Lan 1,BAI Jun jie 1,DENG Guo cheng 1,HUANG Zi ran 2 (1.Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China; 2.South China Agricultural University ,Guangzhou 510640,China);Preparation of recombinant antimicrobial peptides and its antibacterial effects on fish-pathogenic bacteria[J];Journal of Fishery Sciences of China;2002-02
China Proceedings of conference Full-text Database 2 Hits
1 Huang Zi Ran Zhang Wei Min Huang Ya Dong;Research on the Cecropin from Chinese oak silkworm in China over last 30 years[A];[C];2009
2 SUN Zhenjun (Department of Ecology, China Agricultural University, Beijing, 100193, China);Advance research on earthworm antibacterial peptides[A];[C];2010
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 DENG Qiang et al(College of Food Science and Technology,Shanghai Fisheries University,Shanghai 200090);Gene Cloning and Plasmid Construction of Recombinant Bovine Lactoferricin[J];Journal of Anhui Agricultural Sciences;2008-11
2 HUANG Yadong ZEN Jingxiu WEN Shuoyang WANG Yan LI Xiaokun ZHONG Yangjin CAO Yang ( Biopharmaceutical R & D Center of Jinan University, Guangzhou 510632 ;Laboratory of Sericulture Molecular Biology and Biotechnique, South China Agriculture University, Guangzhou 510642 ;3 Laboratory of Insect Ecology, South China Agriculture University, Guangzhou 510642);Synthesis, Cloning and Expression of the Gene of Antifungal Peptide Thanatin-CAD[J];Acta Sericologica Sinica;2003-02
3 GAN Xiao-qin,WU Su-hua,LUO Xiang-dong,YANG Cheng,WU Yan(State Key Laboratory of Trauma,Burns and Combined Injury,Institute of Burns,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Prokaryotic expression and purification of human beta defensin 4 and its bactericidal activity against Pseudomonas aeruginosa[J];Acta Academiae Medicinae Militaris Tertiae;2009-10
4 Zhang Yali, Yang Guoqing, Guo Ying, Chen Yongfu (National Laboratory for Agribiotechnology, China Agricultural University, Beijing 100094);Expression of Optimized-code Bull Protamine in E.coli.[J];High Technology Letters;2002-04
5 ZHOU Lei,ZHANG Yong,WANG Jian-feng,ZHAO Xing-xu(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China);Cloning and prokaryotic expression of beta-defensin 5 gene in dairy cattle[J];Journal of Gansu Agricultural University;2009-01
6 SUN Dong-bo,LIN Yun-cheng,GUO Dong-hua,WANG Jian-fa,ZHANG Min,LIN Yu-cai,WU Rui(College of Animal Science and Technology,Heilongjiang August First Land Reclamation University,Daqing 163319,China);Synthesis of Bovine Lactoferricin B Gene and Construction of Eukaryotic Expression Vector[J];China Animal Husbandry & Veterinary Medicine;2009-05
7 LI Bing,WANG Yu-guang(Tropical Biotechnology Research Institute,Chinese Academy of Tropical Agricltural Sciences,Haikou,Hainan 571101,China);Expression and purification of antibacterial peptide CMIV in Escherchia coli[J];Guangxi Agricultural Sciences;2008-04
8 GUO Guo1,WU Jianwei1,FU Ping1,QIN Ronggui2(1.Department of Parasitology,Guiyang Medical College,Guiyang 550004,China;2.School of Pharmocology,Guiyang Medical College,Guiyang 550004,China);Effects of Antibacterial Peptides from Housefly Larval Secretion on Levels of Serum Lysozyme in Mice[J];Journal of Guiyang Medical College;2007-06
9 Zheng Qing Bao Shixiang Yao Ruhua Huang Ziran * Zheng Xueqin ** Wang Yuguang ** (Dept. of Bioengineering, South China univ. of Tech., Guangzhou 510641);THE DESIGN AND SYNTHESIS OF A NOVEL CECROPIN GENE AND ITS EXPRESSION IN SACCHAROMYCES CEREVISIAE (Ⅱ) ——Expression of Cecropin AD in S.cerevisiae[J];JOURNAL OF SOUTH CHINA UNIVERSITY OF TECHNOLOGY(NATURAL SCIENCE);1998-04
10 Cai Qingsong, Zhang Yan, Zhai Chaoyang. Laboratory of Medical Molecular Biology, West China School of Preclinic and Forensic Medicine, Sichuan University, Chengdu 610041, China;Design of a Series of New Antibacterial Peptide Molecules Based on Computer Structure-function Analysis Software[J];Journal of West China University of Medical Sciences;2002-03
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 MENG Fang,CAO Jin,TIAN Hong,DAI Wan-run,GAO Xiang-dong(School of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,China);Optimization of Engineering Strain BL21(DE3) Expressing Tumor Necrosis Factor Receptors 1 PLAD[J];Pharmaceutical Biotechnology;2012-04
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Li Wen-chu Lu Keng-ming Huang Zi-ran (South China Agricultural University,Guangzhou 510642 ,China) Dai Zhu-ying (Nanjing Normal University, Nanjing 21 0024 ,China) Huang Da-nian (China Natumal Rice Research Institute, Hangzhou 3 1 0003,China);STUDIES ON THE BACTERICIDAL EFFECT OF ANTIBACTERIAL PEPTIDE D FROM CHINESE OAK SILKWORM,ANTHERAEA PERNYI[J];Acta Sericologica Sinica;1991-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved