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《Journal of Anhui Agricultural Sciences》 2008-03
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Genetic Diversity Analysis of 25 Tobacco Germplasm Resources by ISSR Molecular Marker

FU Bing et al(Biotechnology Research Center of Kunming University of Science and Technolgy,Kunming,Yunnan 650224)  
[Objective] The aim was to use ISSR molecular marker to study the genetic adversity of tobacco.[Method]The genetic diversities of 25 tobacco germplasm resources were analyzed by ISSR molecular marker.[Result] 5 primers were screened out from 50 ISSR primers totally to amplify the tobacco genome DNAs of 25 materials effectively and 100 sites were amplified out totally,including 76 polymorphism sites with polymorphism rate of 76%.The Jaccard genetic similarity coefficients among tobacco germplasms were calculated by using NTSYSpc-2.10e software.The genetic similarity coefficients among 25 tobacco germplasms were between 0.313 and 0.938 and the average genetic similarity coefficient was 0.737.The similarity coefficient between wild and cultivated tobaccos was lower,indicating that there was higher genetic diversity between them.The systematical cluster analysis conducted with UPGMA showed that 25 tobacco germplasm resources could be divided into 2 groups,3 wild tobaccos gathered to be a category and 22 cultivated tobacco germplasms gathered to be another big category.[Conclusion] The genetic diversity of tested cultivated tobacco was relatively low and it was urgent to develop genetic base of cultivated tobacco.
【Fund】: 云南省自然科学基金(2004C0006R);; 教育部留学回国人员科研启动基金(2005-383);; 云南省中青年学术技术带头人后备人才培养费(2006y01-10);; 昆明理工大学学生课外学术科技创新基金资助
【CateGory Index】: S572
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