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《Journal of Anhui Agricultural Sciences》 2008-03
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Preparation of Monoclonal Antibodies against HA of H9N2 AIV and Identification of its Subtypes

ZHAN Ai-jun et al(Technical Center of Animal and Plant Quarantine Inspection,Shenzhen CIQ,Shenzhen,Guangdong 518010)  
Balb/C mice were immunized with pcDNA-H9 gene vaccine,purified H9N2 subtype AIV or recombinant bacteriophage T4-H9.After fusion of the splenocytes obtained from the immunized mice with myeloma cells,HI and ELISA screening were done to select hybridoma cell lines,producing five H9 subtype AIV specific monoclonal antibodies H9-01(2E6),H9-02(3B1),H9-03(3H11),H9-04(1A4) and H9-05(2G9).Testing for the subgroup of the monoclonal antibodies revealed that H9-01,H9-02 and H9-05 belonged to the subgroup IgG2b,H9-03 to IgG1,H9-04 to IgG2a.All their light chains were of the kappa type.Specificity testing and homologous virus reaction spectrum testing proved that they were all HA-specific monoclonal antibodies.This provided a base for further study on structure and function of H9 subtype AIV to develop the diagnostic kit to detect it.
【CateGory Index】: S852.5
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