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《Journal of Anhui Agricultural Sciences》 2008-04
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Study on the Optimum Extraction Condition of Gypenosides

SHEN Hong-wei et al(College of Biological & Food Engineering,Dalian Polytechnic University,Dalian,Liaoning 116034)  
[Objective] The purpose was to confirm the optimum condition of extracting gypenosides.[Method] The standard curve was prepared and vanillin-perchloride acid colorimetry was used to determine gypenosides content.The influences of extraction times,extraction temperature,ethanol concentration and material-liquid ratio with different levels on the extraction rate of gypenosides were studied through orthogonal test.[Result] The influences of extraction times,extraction temperature,ethanol concentration and material-liquid ratio on the extraction rate of gypenosides minished in order.The influences of extraction temperature and extraction times on gypenosides were obviously higher than that of ethanol concentration and material-liquid ratio.The intuitive analysis showed that the optimum extraction technology was with 70% ethanol and material-liquid ratio of 1∶12,extracting for 3 times and 2 d once at 40 ℃.F test showed that the effects of extracting gypenosides had no significant difference among 3 ethanol concentration levels and 3 material-liquid ratio levels,but the differences among various levels of temperature and extraction times were significant.[Conclusion] In the extraction course of gypenosides,the difference of extracting conditions had very big influence on the extraction rate of gypenosides.
【Fund】: 国家自然科学基金项目(20476017);; 辽宁省高等学校优秀人员支持计划项目(RC204205);; 辽宁省科学技术基金项目(20042132)
【CateGory Index】: R284;TQ461
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【References】
Chinese Journal Full-text Database 3 Hits
1 Zhang Yanxia, Shi Lingling, Dai Xiangchen, Neo Christophine Mokgolodi, Liu Yujun(College of Biological Sciences and Biotechnolgy,Beijing Forestry University,Beijing 100083,China);Jasmonic acid and its derivatives:a type of elicitors for plant secondary metabolisms[J];Journal of Hainan Normal University(Natural Science);2008-03
2 HUANG Shan,GONG Yan-ling,JIN Hong(College of Chemical Engineering,Qingdao University of Science and Technology,Qingdao 266042,China);Optimization of extraction conditions of gypenosides by enzymatic method[J];Science and Technology of Food Industry;2009-04
3 PIAO Xiang-lan,WU Qian(Institute of Chinese Minority Traditional Medicine,Minzu University of China,Beijing 100081,China);Progressive Studies on Gynostemma pentaphyllum[J];Lishizhen Medicine and Materia Medica Research;2010-07
【Citations】
Chinese Journal Full-text Database 6 Hits
1 HOU Hui-li,FU Tong-sheng(College of Animal Medicine,Hunan Agricultural University,Changsha,Hunan,410128,China);Research Advance in the Chemical Ingredients and Pharmacological Effects of Gynostemma pentaphyllum[J];Progress in Veterinary Medicine;2006-S1
2 MA Ping-bo~1,ZHU Quan-hong~2,HUANG Zhong-wei~1(1.Department of Pharmacy,Nanfang hospital,Southern Medical University,Guangzhou 510515,China;2.Department of TCM,Southern Medical University,Guangzhou 510515);Effects of Gynostemma pentaphyllum on the blood—lipid and hemorheology in hyperlipidemia rabbits[J];The Chinese Journal of Modern Applied Pharmacy;2005-06
3 Chen Jianxiong;Liao Duan fang;Tang Xiaoqing;et al (Address:Chen Jianxiong,Department of Pharmacology,Hengyang Medical College,Hengyang);Protection of Gypenoside Against Oxygen Free Radical Induced Vasospasm of Isolated Rabbit Basilar Artery[J];CHINESE TRADITIONAL AND HERBAL DRUGS;1997-04
4 Chen Shu-kun Kunming Institute of Botany, Academia Sinica, Kunming 650204;A CLASSIFICATORY SYSTEM AND GEOGRAPHICAL DISTRIBUTION OF THE GENUS GYNOSTEMMA BL.(CUCURBITACEAE)[J];Acta Phytotaxonomica Sinica;1995-04
5 Feng Binghong, Meng Qing, Guo Xiaoling, et al. (Dept of Pharmacology, Guangdong College of Pharmacy, Guangzhou 510224);Effects of Gynosaponin SH-6 on Monamines and Their Metabolites in Rat Brain[J];TRADITIONAL CHINESE DRUG RESEARCH & CLINICAL PHARMACOLOGY;1998-02
6 Wang Bin,Ge Zhidong,Zhou Aiwu,et al. Clinical Pharmacology Institute,Anhui Medical University,Hefei 230032;Effects of Gypenosides on Immune Function of Rats in Vitro[J];TRADITIONAL CHINESE DRUG RESEARCH & CLINICAL PHARMACOLOGY;1999-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Sun Rongrong,Xiao Yaping,Cheng Xiaoxia(1.Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry,Xi'an 710062,Shanxi,China;2.Life Science College,Shanxi Normal University,Xi'an 710062,Shanxi,China);Contents of Total Flavonoids and Activity of PAL in Gynostemma after Adding Licylic Acid[J];Anhui Agricultural Science Bulletin;2008-09
2 ZHANG Jin,WANG Chang-jun,XIE Jiang,CHEN Fang(Faculty of Pharmacology,Bengbu Medical College,Bengbu 233030,China);Protective effect of gypenosides on adriamycin induced acute cardiotoxicity in rats[J];Journal of Bengbu Medical College;2007-04
3 WAN Li, WAN Xing-Wang, HU Jin-Hong (1. Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2. International Cooperation Laboratory of Signal Transduction, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438);Effect of gypenosides on hepatic function and fibrosis in rats with immune fibrosis[J];Academic Journal of Second Military Medical University;2003-12
4 LIN Ru 1,2 ,CAO Yu fang 2,HU Zheng hai 2* (1 College of Life Science,Fujian University of Agriculture and Forestry,Fuzhou 350002,China;2 Institute of Botany,Northwest University,Xian 710069,China);Anatomical structure of vegetative organs and histochemical localization of ginsenosides in Gynostemma pentaphyllum[J];Acta Botanica Boreali-occidentalia Sinica;2002-04
5 LIN Ru 1.2 , LIU Shi biao 1,CAO Yu fang 1,HU Zheng hai 1* (1 Institute of Botany, Northwest University, Xi'an 710069 China; 2 College of Life Science, Fujian University of Agriculture and Forestry, Fuzhou 350002 China);Studies on the correlation between the content of gypenosides and its vegetative organs and growth period[J];Acta Botanica Boreali-occidentalia Sinica;2003-07
6 HOU Hui-li,FU Tong-sheng(College of Animal Medicine,Hunan Agricultural University,Changsha,Hunan,410128,China);Research Advance in the Chemical Ingredients and Pharmacological Effects of Gynostemma pentaphyllum[J];Progress in Veterinary Medicine;2006-S1
7 LIU Shi-biao~1,LIN Ru~2,HU Zheng-hai~3(1.Institute of Ecology,Jishou University,Jishou,Hunan 416000,China;2.College of Life Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;3.Institute of Botany,Northwest University,Xi′an,Shaanxi 710069,China);Comparison of stem and leaf structures and total gypenosides among 5 species of Gynostemm[J];;2006-05
8 LIU Lu-jiong,WANG Xiao-long (Department of Cardiology,Shuguang Hospital affiliated with Shanghai University of Traditional Chinese Medicine, Shanghai 201203,China);Applications of heat-clearing and detoxicating method in the treatment of coronary heart disease[J];World Clinical Drugs;2009-09
9 LIU Hai,YANG Yu,JIN Feng-xie,YU Hong-shan(School of Biological & Food Engineering,Dalian Polytechnic University,Dalian 116034,China);Isolation and identification of gypenoside in gypenoside hydrolysate[J];Journal of Dalian Polytechnic University;2010-02
10 Zheng Xiaojiang; Liu Jinlong(Hubei Institute for Nationalities,Enshi 445000);ON THE RESEARCH AND DEVELOPMENT OF CYNOSTEMMA PENTAPHYLLUM [(THUMB)MAKINO][J];JOURNAL OF HUBEI INSTITUTE FOR NATIONALITIES;1997-06
China Proceedings of conference Full-text Database 4 Hits
1 Feng Xu,Yang Zhang, Ying He, Jingcai Li (Shenyang Pharmaceutical University Chronopharmacology, Shenyang Liaoning, 110016);Potentiating effects of melatonin on the immune functions in aging mice[A];[C];2004
2 He Ying,Li Jingcai,Zhang Yang,Xu Feng. College of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016;Effects of melatonin on the immune functions in aging animals[A];[C];2004
3 Yang Zhang, Ying He, Feng Xu, Jingcai Li (Shenyang Pharmaceutical University Chronopharmacology, Shenyang Liaoning, 110016);Comparision of Effects of Melatonin,Thymosin and Interleukin 2 on the Immune-modulating Function and the Mechanisms of Immune-modulating Function of Melatonin[A];[C];2004
4 HE Ying,LI Jing-Cai,ZHANG Yang,et al(School of Pharmacy,Shenyang Pharmaceutical University,Liaoning Shenyang 110016);Immune-modulating function of melatonin and it’s mechanisms[A];[C];2004
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHOU Yu-bi et al (Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,Qinhai 810001);Effect of BSA on RAPD Analysis of Cynomorium songaricum[J];Journal of Anhui Agricultural Sciences;2007-28
2 ZHANG Jin,WANG Chang-jun,XIE Jiang,CHEN Fang(Faculty of Pharmacology,Bengbu Medical College,Bengbu 233030,China);Protective effect of gypenosides on adriamycin induced acute cardiotoxicity in rats[J];Journal of Bengbu Medical College;2007-04
3 WANG Li~(1,2);ZHANG Yan-xia~1;SHI Ling-ling~1;LIU Yu-jun~1.1 College of Biological Sciences and Biotechnology,Beijing Forestry University,100083,P.R.China;2 Department of Medicine,Tibet Institute for Nationalities,Xianyang City,Shaanxi Province,712082,P.R.China.;Application of functional genomic and metabolomic techniques to the studies on biosynthesis and regulation of plant secondary metabolites[J];Journal of Beijing Forestry University;2007-05
4 LIN Ru 1.2 , LIU Shi biao 1,CAO Yu fang 1,HU Zheng hai 1* (1 Institute of Botany, Northwest University, Xi'an 710069 China; 2 College of Life Science, Fujian University of Agriculture and Forestry, Fuzhou 350002 China);Studies on the correlation between the content of gypenosides and its vegetative organs and growth period[J];Acta Botanica Boreali-occidentalia Sinica;2003-07
5 LI Junji1,LIAO Hefa2,LIU Hailong1,3 (1.Guangxi Forestry Research Institute,Nanning 530001;2.Dawu County Forestry Department,Hubei Province 432800;3.Life Science College of Zhejiang University,Hangzhou 310029);Extract Method of DNA Genome of a Medical Plant Dysosma pleiantha[J];Guangxi Forestry Science;2007-02
6 CHEN Li,WEI Li*,ZHOU Tong*,LI Min-Yu*,QIN Yu-Bin*,WU Yao-Sheng **(Department of Biochemistry and Molecular Biology,Guangxi Medical University,Nanning 530021,China);Study on isolation methods of genomic DNA in several Chinese herbal medicines[J];Guihaia;2007-01
7 Wang Manli Wu Yinghua (Department of Chemical Engineering) Chen Tong (Physical and Chemical Analysis Center);EXTRACT OF GYNOSAPONIN BY COLUMN CHROMATOGRAPHY[J];JOURNAL OF GUIZHOU UNIVERSITY OF TECHNOLOGY;1997-03
8 DONG Hao di, ZHONG Jian jiang * (State Key Laboratory of Bioreactor Engineering ECUST, Shanghai 200237, China)$$$$;Enhancement of Taxane Production in Suspension Cultures of Taxus chinensis with Elicitation[J];Journal of East China University of Science and Technology;2002-01
9 LUO Zhi yong, ZHOU Gang, CHEN Xiang hui, et al (Molecular Biology Research Center, Xiangya Medical College, Central South University, Changsha 410078, China);Isolation of high-quality genomic DNA from plants[J];Bulletin of Hunan Medical University;2001-02
10 Deng.Shilin;Li Xinfeng;ChenBenmei;Deng FuliangAnalytical Testing Center,Hunan Medical UniversityZhou XiaojuanEnvironment Medical heboratory,Hunan Medical University;ANALYSIS OF AMINO ACIDS,VITAMINS ANDCHEMICAL ELEMENTS IN GYNOSTEMMAPENTAPH YLLU M (TH UN B)MAKINO[J];BULLETIN OF HUNAN MEDICAL UNIVERSITY;1994-06
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 CHEN Gong-de,HE Ri-liu,CHEN Jie,DAI Qing,CUI Peng (School of Chemical Engineering,Hefei University of Technology,Hefei 230009,China);Study on extraction technology of toosendanin by enzymatic hydrolysis[J];Applied Chemical Industry;2010-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhang Zhi(Department of Biological Engineering of Anhui Agricultural University,Hefei 230036 );A New Spccics of Gynostemma from China[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1995-02
2 Chen Xiuxiang and Liang Dingren (Guangxi Institute of Ethnical Medicine and Pharmacology, Manning 530001);A NEW SPECIES ON MEDICINAL PLANTS OF GENUS GYNOSTEMMA FROM GUANGXI[J];Guihaia;1991-01
3 Zhang Ruizhe,Traditional Chinese Medicine Related Company,Heilongjiang(150056);Research Progress in Pharmacology and Clinical Of Gynostemma Pentaphyllum(Thunb)Maxcino[J];Heilongjiang Medical Journal;2000-05
4 Han Xiaoyan; Wei Hong-bo; Lian Jan-xue et al(Department of pharmacology,Medical College,LouYang 471003);THE EFFECTS OF GYPENOSIDE ON EXPERIMENTAL HYPERLIPIDEMIA IN RATS[J];HENAN MEDICAL RESEARCH;1996-02
5 Ding Jian-nan (Jiangxi Academy of Sciences. Nanchang, Jiangxi);A NEW VARIETY OF GYNOSTEMMA YIXINGENSE[J];Bulletin of Botanical Research;1990-03
6 Ding Gui-feng Xi Hong-li Deng Yu-Ian Deng Hong-ye Shang Hong-sheng Department of Immunology,Beijing Medical University;MEASUREMENT OF IL-2 BY ACTIVATING MOUSE SPLEEN CELLS[J];;1988-01
7 LUO Dian-hui,WANG Zhao-jing Departmeng of Bioengineering and Biotechnology,Huaqiao University,Quanzhou 362021,China;Research Advances in Polysaccharide of Gynostemma pentaphyllum Makino[J];Letters in Biotechnology;2005-06
8 MA Ping-bo~1,ZHU Quan-hong~2,HUANG Zhong-wei~1(1.Department of Pharmacy,Nanfang hospital,Southern Medical University,Guangzhou 510515,China;2.Department of TCM,Southern Medical University,Guangzhou 510515);Effects of Gynostemma pentaphyllum on the blood—lipid and hemorheology in hyperlipidemia rabbits[J];The Chinese Journal of Modern Applied Pharmacy;2005-06
9 Li Lin & Jin Youyu (Dept. of Pharmacology, Capital Institute of Medicine, Beijing);THE INFLUENCE OF JIAOGULAN EXTRACT ON PLATELET AGGREGATION AND ARACHIDONATE METABOLISM IN RABBITS[J];Chinese Pharmacological Bulletin;1989-04
10 IU Li-Bo,HU Bi,LIAO Duan-Fang1 (Dept of Physiology; 1Dept of Pharmacology, Hengyang Medical College,Hunan 421001);Protective effect of gypenosides on free radical damage of isolated guinea pig papillary muscles[J];Chinese Pharmacological Bulletin;1993-04
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