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《Journal of Anhui Agricultural Sciences》 2008-05
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Primary Exploration on the Extraction Technology of Crude Superoxide Dismutase from Chinese Quince

CAI Zhi-hua(Institute of Bio-pharmacies,College of Life Science,Chongqing Normal University,Chongqing 400047)  
[Objective] The purpose of the study was to screen out the optimum extraction technology of crude superoxide dismutase(SOD) in Chinese quince.[Method] With Qijiang Cheanomeles speciosa from Chongqing as material,SOD dissolution,impure protein removal and SOD sedimentation as basic technology process,4 extraction technologies of SOD was studied comparatively.And the detection results on unit protein,activity and specific activity of SOD extracted by the 4 technologies were compared.[Result] The dosage of phosphate buffer solution had direct influence on the extraction effect,and the determined highest specific activity was 54.55 U/mg when the ratio of buffer solution to Chinese quince was 3∶1.The impure protein removal effect in crude enzyme solution adding 2.5 time volumes of chloroform-ethanol mixed solution was more obvious.The SOD sedimentation effect of in crude enzyme solution added(NH4)2SO4 till 90 % saturation was better and the specific activity increased 44.52 U/mg in comparison with extraction solution.[Conclusion] The optimum technological condition of extracting crude SOD from Chinese quince was temperature of 25~30 ℃,pH value of 7.8,ratio of 0.05 mol/L phosphate buffer solution to Chinese quince of 3∶1,removing impure protein with 2.5 time volumes of chloroform-ethanol mixed solution and precipitating SOD by adding 55 %(NH4)2SO4 till 90 % saturation.
【Fund】: 重庆市扶贫办科技扶贫项目资助
【CateGory Index】: Q814.1
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