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《Journal of Anhui Agricultural Sciences》 2008-06
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Determination of Benzoic Acid,Sorbic Acid and Saccharin Sodium in Fruit Can by HPLC

ZHA Yu-bing et al(Institute of Agricultural Product Processing,Chinese Academy of Tropic Agricultural Sciences,Zhanjiang,Guangdong 524001)  
[Objective] The study aimed to establish the HPLC method of determining benzoic acid,sorbic acid and saccharin sodium in fruit can. [Method] The HPLC method of determining benzoic acid,sorbic acid and saccharin sodium in fruit can was established through recovery,precision and repeatability experiments.[Result] The wavelength of 230 nm was confirmed to be determination wavelength through ultraviolet scan.In the range of 0.010~0.20 g/L,the correlation coefficients of regression equations of benzoic acid,sorbic acid and saccharin sodium were bigger than 0.99 with detection limits of 0.010,0.004 and 0.002 g/L resp.The recoveries of benzoic acid,sorbic acid and saccharin sodium in fruit can were in 94.0%~102% with variation coefficients in 0.56%~2.15%.The RSDs of peak areas of benzoic acid,sorbic acid and saccharin sodium in 5 parallel determinations by sample-determination method were 0.92%,0.79% and 0.95% resp.[Conclusion] Determination of benzoic acid,sorbic acid and saccharin sodium in fruit can by this method had good accuracy and precision.
【CateGory Index】: TS297
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