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《Journal of Anhui Agricultural Sciences》 2008-06
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Establishment of PCR Method for Diagnosing Actinobacillus pleuropneumoniae Strains in Jiangxi Province

LU Xing-hua(Jiangxi Biotechnology Occupation Academy,Nanchang,Jiangxi 330200)  
In the suspected of Actionbacillus pleuropneumoniae(APP)typical cases of illness pigs from the Nanchang area of Jiangxi,gathered sickness pig's pathological change organization,obtained three separation strains which named JXAV-AIII0611,JXAV-AVII0611,JXAV-AX0611 after the bacterium separation.By way of smearing dyeing microscopic exam,the growing character observation,the biochemistry identification as well as the medicine sensitive experiment,confirmed this pathogenic bacteria was Actionbacillus Pleuropneumoniae(APP) Meanwhile using the APP oml gene designed primer,successed in increasing the anticipated size of the gene fragment by the Polymerase chain reaction(PCR),lay a certain foundation for the rapid diagnosis of the disease.
【CateGory Index】: S852.61
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