Primary Report on the Establishment of Regeneration System of Euonymus japonicus cv.CuZhi and Its Rapid Propagation
MA Jie et al(Institute of Biotechnology,Zhengzhou Teacher's College, Zhengzhou,Henan 450044)
[Objective] The study aimed to explore the method and condition for establishing the regeneration system of Euonymus japonicus cv CuZhi.[Method] With MS as basic medium,the tender leaves and axillary buds of CuZhi as materials,the media added 6-BA and NAA with different concentration proportions and the media added IAA with different concn.were set up to conduct the cultures of inducing adventitious buds,proliferation and rooting.[Result] MS could be as basic medium for the regeneration system of CuZhi.The combination of 6-BA and IAA was in favor of inducing calli and adventitious buds from leaves and axillary buds,the effect of medium MS+6-BA 1.0 mg/L+NAA 0.1 mg/L was best and the induction rate of axillary buds was up to 66.67%.The combination of 6-BA with higher concentration and NAA with lower concentration was in favor of the proliferation of clustered buds,the proliferation effect of medium MS+6-BA 1.0 mg/L+NAA 0.2 mg/L was more perfect and the proliferation rate was up to 328.57%.The optimum rooting effect could be obtained from medium MS + IAA 0.5 mg/L + 3% sucrose or 1/4MS + IAA 0.5 mg/L + 2% sucrose.[Conclusion] The establishment of the system provided reference basis for the rapid propagation of CuZhi seedlings.