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《Journal of Anhui Agricultural Sciences》 2008-06
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DNA Extraction from Oil Sunflower Seeds and the Optimization of Its RAPD Reaction System

XUE Zhi-zhong et al(College of Biological Science and Engineering,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018)  
[Objective] The research aimed to lay the foundation for the genetic relationship analysis of oil sunflower and hybrid identification by RAPD technology.[Method] With 6 varieties of hybrid oil sunflower seeds as tested materials,genomic DNA were extracted from oil sunflower by the improved CATB method and the concentration and purity of the extracted DNA were determined.Through single-factor gradient test,the optimized and stable RAPD-PCR raction system was screened out.[Result] DNA extracted from oil sunflower by the improved CATB method had no pollution of protein and phenols,with good quality and high yield.The optimum RAPD-PCR raction system was as follows: 2.0 μl 10×Buffer,2.0 mmol/L Mg2+,150 μmol/L dNTPs,1.5 U Taq enzyme,2.0 ng/μl DNA template and 0.25 μmol/L primers with total volume of 20.0 μl.6 oil sunflower hybrids with different oil content were amplified by this system and they all showed clear and stable bands type with suitable band number.[Conclusion] This RAPD reaction system was suitable for DNA analysis of all kinds of oil sunflower hybrids.
【Fund】: 河北科技大学大学生科技创新基金项目
【CateGory Index】: S565.5
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