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《Journal of Anhui Agricultural Sciences》 2008-06
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Study on the Detoxification Technology of Lilium longiflorum Thumb

YANG Bo-yun et al(College of Life Science,Nanchang University,Nanchang,Jiangxi 330047)  
[Objective] The aim of the research was to discuss the optimum approach for producing the virus-free seedlings of Lilium longiflorum Thumb.[Method] With aseptic seedlings and bulblets of L.longiflorum as tested materials,the correlation between shoot tip size and source and the survival rate and the detoxification rate were studied.And the effects of high temperature pretreatment,pretreatment with chemical inhibitor ribavirin,etc.on the survival rate of shoot tips and the detoxication effect were also analyzed.[Result] There was a negative correlation between the meristematic size of shoot tips and the detoxication rate.The bigger the meristem was,the lower the detoxication rate was and the higher the induction rate was.The inhibition of high temperature on 4 kinds of virus in turn was CMV,LSV,TBV and LRV.Some concn.of ribavirin could efficiently passivate the virus and increase the detoxication rate.Single detoxication treatment was difficult to remove the virus completely,or that needed longer time.[Conclusion] Using combination of 2 or 3 kinds of detoxification technologies was the primary method for producing the virus-free seedlings of L.longiflorum.
【Fund】: 项目基金江西省科技厅国家教育部资助项目
【CateGory Index】: S644.1
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