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《Journal of Anhui Agricultural Sciences》 2008-07
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RAPD Analysis on 2 Species of Northern Medicinal Materials

ZHANG Ai-hua et al (College of Traditional Chinese Medicinal Materials,Jilin Agricultural University,Changchun,Jilin 130118)  
[Objective] The aim of the research was to provide the basis for the identification of Chinese medicinal materials at molecular level.[Method] Genomic DNA was extracted from A.sieboldii Miq.var.seoulense Nakai,Asarum heterotropoides Fr.Schmidt var.mandshuricum(Maxim.) Kitag.,Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hsiao and Astragalus membranaceus(Fisch.) Bge.200 random primers were used to make RAPD amplification and the genetic relationships between the medicinal materials were analyzed through clustering analysis.[Result] 129 primers had amplified products,occupying 64.5% of total primer number and their sizes of amplified fragments were 250~2 000 bp.19 primers that amplified clear bands and had good repeatability were screened out.The results of clustering analysis showed that the intraspecific comparability was greater than the interspecific comparability.A.sieboldii and A.heterotropoides had closer genetic relationship,while Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hsiao and Astragalus membranaceus(Fisch.) Bge.had closer genetic relationship.[Conclusion] This research proved that A.sieboldii and A.heterotropoides,Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hsiao and Astragalus membranaceus(Fisch.) Bge.were different species of medicinal materials and there was the interspecific difference between asarum and Huangqi.
【CateGory Index】: R282
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【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 CHEN Yongjiu~1 WANG Wen~1 YANG Yuexiong~2 SU Bing~1 ZHANG Yaping~1 XIONG Luyun~3 HE Zhaofan~3 SHU Chuang~2 YANG Darong~2 (1 Laboratory of Cellular and Molecular Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223) (2 Laboratory of Insect Resources Chinese Academy of Sciences Kunming 650223) (3 Institute of Biochem and Nutriology Guizhou Agricultural College Guiyang 550025);Genetic Divergence of Cordyceps sinensis as Estimated by Random Amplified Polymorphic DNA Analysis[J];Acta Genetica Sinica;1997-05
2 Hui Dongwei; Zhuang Bingchang;Chen Shouyi(Institute of Genetics,Academia Sinica,Beijing 100101)(Institute of Soybean Research, Jiling Academy of Agriculture Sciences Gongzhuling 136100);Phylogeny of Genus Glycine Reconstructed by RAPD Fingerprinting[J];ACTA GENETICA SINICA;1996-06
3 Zhang Rong;Shao Jienben;Tian Xueming(Department of Pharmacy,Kunming General Hospital,Chengdu Military Region, Kunming);Identification of Seven Species of Clematis(Clematis L.)by RAPD Analysis[J];Chinese Traditional and Herbal Drugs;1996-11
4 任桂珍,孔繁荣,张耕耘,曹玉璞,张佳杰,朱学骏,陈受宜;CLONING AND SEQUENCING OF THE MOMP GENE VS4 DNA OF CHLAMYDIATRACHOMATIS AND THE APPLICATION IN THE STUDY OF VERTICAL TRANSMISSION[J];Chinese Journal of Microbiology and Immunology;1995-01
5 Wang Xiao quan, Zou Yu ping, Zhang Da ming, Zhang Zhi xian and Hong De yuan (Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Academia Sinica, Beijing 100093);PROBLEMS IN THE USE OF RAPD TO THE STUDY OF GENETIC DIVERSITY AND SYSTEMATICS[J];Acta Botanica Sinica;1996-12
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