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RAPD Analysis on 2 Species of Northern Medicinal Materials

ZHANG Ai-hua et al (College of Traditional Chinese Medicinal Materials,Jilin Agricultural University,Changchun,Jilin 130118)  
[Objective] The aim of the research was to provide the basis for the identification of Chinese medicinal materials at molecular level.[Method] Genomic DNA was extracted from A.sieboldii Miq.var.seoulense Nakai,Asarum heterotropoides Fr.Schmidt var.mandshuricum(Maxim.) Kitag.,Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hsiao and Astragalus membranaceus(Fisch.) Bge.200 random primers were used to make RAPD amplification and the genetic relationships between the medicinal materials were analyzed through clustering analysis.[Result] 129 primers had amplified products,occupying 64.5% of total primer number and their sizes of amplified fragments were 250~2 000 bp.19 primers that amplified clear bands and had good repeatability were screened out.The results of clustering analysis showed that the intraspecific comparability was greater than the interspecific comparability.A.sieboldii and A.heterotropoides had closer genetic relationship,while Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hsiao and Astragalus membranaceus(Fisch.) Bge.had closer genetic relationship.[Conclusion] This research proved that A.sieboldii and A.heterotropoides,Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hsiao and Astragalus membranaceus(Fisch.) Bge.were different species of medicinal materials and there was the interspecific difference between asarum and Huangqi.
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