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《Journal of Anhui Agricultural Sciences》 2008-07
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Research on the In Vitro Bacteriostatic Effect of Quassiawood Alkaloid on Escherichia coli

HE Ying et al (Guangxi Veterinary Institute,Nanning,Guangxi 530001)  
[Objective]The aim of the study was to determine the in vitro bacteriostatic effects of quassiawood alkaloid on Escherichia coli strains C249,WM and YL.[Method]The plate culture method was used to prepared the bacterium solution,the micro-dilution method was used to determine the minimum inhibitory concn.(MIC) and the cylinder plate method was used to conduct in vitro bateriostasis experiment.[Result]The fat-soluble quassiawood alkaloids had stronger inhibitory effect on E.coli in vitro,its bacteriostatic circle diameters on E.coli strains C249,WM and YL were 19.07±0.23,20.77±0.59 and 23.00±0.21 mm resp.,belonging to high sensitivity and its MICs were 3.2,1.6 and 1.6 mg/ml resp.The water-soluble quassiawood alkaloids hardly had bacteriostasis on the above strains and its bacteriostatic circle diameters were smaller than 13 mm,belonging to low sensitivity.[Conclusion]The in vitro bacteriostatic effect of fat-soluble quassiawood alkaloids on the standard highly virulent strain of E.coli was superior to berberine sulphate,equal to miquindox and ofloxacin,a little worse than florfenicol,but that on the highly virulent strains WM and YL isolated in Guangxi was obviously superior to berberine sulphate,miquindox and ofloxacin,and equal to florfenicol.
【Fund】: 广西科技攻关资助项目(桂科攻0424001-3A)
【CateGory Index】: R285
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