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《Journal of Anhui Agricultural Sciences》 2008-08
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Study on the Transonic Extraction and Content Determination Methods of Total Saponins of Panax Notognseng( PNS)

QIN Feng et al (JianysuR & D Center of Animal Pharmaceutical, Taizhou, Jiangsu 225300)  
[Objective] The research aimed to optimize the methods of the content determination and transonic extraction process of total saponins of panax notognseng(PNS). [Methods] The content of saponins was determined by the methods of vanillin-perchloric acid and the optimum extraction process was optimized by L9(34) orthogonal design. [Result] The detective wavelength of PNS was 560 nm and the linearity of panax saponin Rg1 was obtained at the range of 0.008 3-0.049 6 mg/ml(r=0.999 4). The average recovery was 99.3% and RSD was 3.4% (n=4) respectively. The optimum transonic extraction conditions were transonic extracted times of 50 minutes each time, temperature of 40 ℃ and solid -liquid ratio of 1∶12. [Conclusion] The method of content determination was simple, feasible, highly accurate and precise. The extraction process was economical and reasonable, deserved popularizing all the more.
【Fund】: 江苏省泰州市资助项目
【CateGory Index】: R284;TQ461
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