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《Journal of Anhui Agricultural Sciences》 2008-08
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Analysis on the Isozymes Changes in the Differentiation Stage of Young Panicle of Isonuclear Alloplasmic Rice Plant

JIN Xi-biao et al (Rice Research Institute, Sichuan Agriculture University, Wenjiang, Sichuan 611130)  
[Objective]The research aimed to reveal the relation between the change of isozyme bonds and Cytoplasmic Male Sterile (CMS) pollen abortion at the differentiation stages of young rice panicle. [Method] Comparative studies on EST, COD and POD isozyme of the leaves and young panicles from CMS line-G46A and its maintainer-G46B at series periods of young panicle differentiation stage were performed by isozyme PAGE electrophoresis analyses. [Result] The results indicated that the bands of EST in G46A both leaves and young panicle were more abundant than those in G46B and the activity of EST wre also higher in G46A. The changes began at pollen mother cell stage, enhanced considerably at one nuclear stage and lasted to three nuclear stage. There was no difference in the bands distribution of COD and POD in the leaves of G46A and G46B, but in young panicle, the bands became more abundant in G46A at one nuclear stage. This change was consistent with the observation that the pollen abortion starts at one nuclear stage. [Conclusion] The loss of some characteristic bands of COD and POD would be as the physiological and biochemical signals of CMS pollen abortion of G46A.
【Fund】: 国家科技支撑计划(2007BAD81B00)
【CateGory Index】: S511
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