Research on Pollen Viability of New Chinese Chestnut Lines
CHEN Zai-xin et al (College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei 434025)
[Objective] The research aimed to supply basis for the selection of pollinated Chinese chestnut varieties in the extension and setting down the technique of promoting fruiting by spraying boron in the flowering period in the production. [Method] With the pollens of 8 new Chinese chestnut lines such as 0203, 0204 and 0205 as tested materials, the determination experiment on the pollen viability of Chinese chestnut of the 2 factors of culture time (0, 6, 12, 18, 24, 30 and 36 h) and boric acid concn. (0.0%, 0.1%, 0.2% and 0.3%) was conducted by germination on solid media. And the viabilities of pollens in different lines were compared under the optimum culture time and boric acid concn. [Result] The boric acid concn. of 0.2% and culture time of 30-36 h were most favorable for the germination of pollens in superior lines and their germination rates were 44.9%-89.0%. The pollen viability had significant difference among different lines, among which the pollen viability of 0213 was strongest, being up to 89.0%, that of 0203, 0204, 0205, 0207, 0208, 0212 and 0213 were secondary, being higher than 60% and that of 0211 was lowest, being only 44.9%. [Conclusion] The addition of 0.2% boric acid solution in solid media had significant promoting effect on pollen viability.