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《Journal of Anhui Agricultural Sciences》 2008-08
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Determination on Swertisin in Different Parts of Swertia franchetiana H.Smith

MA Yu-hua et al (Department ofChemistry, Qinghai Nationalities University, Xining, Qinghai 810007)  
[Objective] The research aimed to determine the content of swertisin in different parts of Swertia franchetiana H.Smith by reversed- phase high performance liquid chromatography. [Method] The analysis was performed on a Kromasil C18 (250 mm×4.60 mm, 5 μm). The mobile phase consisted of CH3OH and 0.02% phosphoric acid in water. The flowrate was 1 ml/min. The detection wavelength was at 260 nm. [Result] The linear range of swertisin was within 0.92- 4.60 μg(r=0.999 5). The average recovery was 98.73%,RSD was 0.31%. [Conclusion] The content ofswertisin was most high in flowers.
【Fund】: 国家中西部重点项目(2001BA901A47)资助
【CateGory Index】: S567.239
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