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《Journal of Anhui Agricultural Sciences》 2008-09
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Distribution of Microsatellite from Complete Sequence of Medicao truncatula Chloroplast DNA

PIAN Rui-qi et al(Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants,Mininstry of Education,Beijing Forestry University,Beijing 100083)  
In order to develop the universal chloroplast simple sequence repeat(cpSSR) primer for Leguminous plant,the publicly available chloroplast DNA sequence of Medicao truncatulawas was analyzed with bioinformatics software.The research content included the abundance and distribution of cpSSR across the Medicao truncatula chloroplast genome.Based on the investigation of mono-to hexa-nulecleotide simple sequence repeat(SSR),341 microsatellitate repeat sequences were observed in whole chloroplast genomic DNA sequence.About 3.21% of the chloroplast genomic DNA was occupied by SSRs,whose total length was 3 987 bp.The distribution of cpSSR was very uneven,mainly concentrated in some area of large single copy(LSC).Among all of repeat sequences,monocleotide repeat was the most abundant,with the number of 297,which was a proportion of 87.1%.The second was the dinucleotide,43(12.6%).However,the trinucleotide repeats was rare,only 1.No repeats which contained more than trinucleotide repeats were found.The porpotion of repeat sequence which was composed of A and T was 41.4% and 55.6%,respectively.The number of AT and TA repeats occupied 95.3% of dinucleotide repeat.Array mode of sequences was divided into three types: perfect repeat,interrupted repeat and compound repeat.The number of interrupted repeats was 192,taking the largest proportion in sequences,64.6%.During monocleotide repeats,the number of perfect and compound repeats was 76(25.6) and 29(9.8%),respectively.These results would be useful for molecular markers' screeing.
【Fund】: “863”国家高新技术项目(2002AA241111)
【CateGory Index】: S541.9
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