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《Journal of Anhui Agricultural Sciences》 2008-10
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Effects of Inoculating Azotobacter on the Physiological Characteristics of Nitrogen Nutrition in the Tissue Cultured Seedlings of Sugarcane

LIANG Jun et al (Guangxi University,Nanning,Guangxi 530004)  
[Objective] The aim was to study the effects of azotobacter inoculation on the physiological characteristics of nitrogen nutrition in the tissue cultured seedlings of sugarcane.[Method] Two species of endophytic azotobacters B16L and B8R were inoculated into to the tissue cultured seedlings of sugarcane cultivars G16,R16 and R22 so as to study the changes of physiological characteristics of nitrogen nutrition in these tissue cultured seedlings.[Result] Determinations in tillering and jointing stages showed that the chlorophyll content and Ca2+-ATPase activity in the +1 leaves of R16 and R22 in treatments were bigger than that in CK and the Ca2+-ATPase activity in the +1 leaf of G16 in treatment was lower than that in CK.Compared with tillering stage,the chlorophyll content and Ca2+-ATPase activity in the +1 leaves of different cultivars in treatments and CK in jointing stage had some increase.The nitrate nitrogen contents in the +1 leaves of different cultivars in treatments and CK changed irregularly.[Conclusion] The inoculation of endophytic azotobacter improved some physiological characteristics of nitrogen nutrition in the healthy tissue cultured seedlings of R16 and R22,but had little effect on G16.
【Fund】: 国家自然科学基金(30260054 3066085);; 广西农业科学院科技发展基金(2004013);; 广西科学研究与开发项目(桂科能0330015-1E);; 广西自然科学基金(桂科基0778006-5 桂科基0448092)
【CateGory Index】: S566.1
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