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《Journal of Anhui Agricultural Sciences》 2008-10
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Study on Gliadin Genetic Trait of Wild Elymus nutans in the Northwestern Plateau of Sichuan Province

ZHANG Jian-bo et al (Guizhou Institute of Prataculture,Dushan,Guizhou 558200)  
[Objective] The aim was to ravel the genetic difference between wild Elymus nutans in the northwestern plateau of Sichuan Province and the materials from the established fingerprinting database.[Method] Fifty-five shares of E.nutans materials collected from northwest plateau of Sichuan Province,Xinjiang,Tibetr and Qinghai were used to establish the gliadin fingerprinting database by A-PAGE technique.The gliadin fingerprints of the materials from northwest plateau of Sichuan Province were compared with that from the established fingerprinting database.[Result] Forty-one bands were separated from materials from northwest plateau of Sichuan Province and 87.80% of them were polymorphic,which was fewer than that from materials from the established fingerprinting database(45 bands and 93.33% of them were polymorphic).The Shannon index,Simpson index and genetic similarity coefficient were also less than that of materials from the established fingerprinting database,suggesting that the gliadin genetic polymorphism level in the materials from northwest plateau of Sichuan Province was lower than the average level.The particular bands(2 bands) of materials from northwest plateau of Sichuan Province accounted for 40% of total bands(5 bands) in fingerprinting database,which showed that the E.nutans in northwest plateau of Sichuan Province had its particular genetic features.[Conclusion] The materials from northwest plateau of Sichuan Province could be clustered into a group and its gliadin map type had some correlation with the altitude at which the materials were.
【Fund】: 四川省科技厅基础研究项目(05jy029-060)
【CateGory Index】: S543.9
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