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《Journal of Anhui Agricultural Sciences》 2008-11
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Study on Genetic Diversity and Nucleus Germplasm Construction of Yongning Common Wild Rice(Oryza rufipogon Griff.) Populations in Guangxi

LIANG Yan-li et al(Guangxi Crop Genetic Improvement and Biotechnology Lab,Agricultural College of Guangxi University,Nanning,Guangxi 530007)  
[Objective] The research aimed to the genetic diversity and nucleus germplasm construction of the wild rice species populations from Yongning in Guangxi,for providing references to properly maintain the population of common wild rice(Oryza rufipogon Griff.).[Method] The genetic diversity of 243 common wile Oryza rufipogon Griff.samples was analyzed with 24 SSR markers selected evenly from 12 chromosomes of rice genome.[Result] The result indicated that all SSR loci were polymorphic with 100% of polymorphic loci(P).A total of 103 alleles was detected at these 24 SSR loci,and the average of alleles per loci(A),effective number of Alleles(E),expected heterozygosity(He) and polymorphism information content(PIC) were 4.291 7,2.511 2,0.573 2 and 0.572 0,respectively.Cluster analysis led to construction of 31 samples of nucleus germplasm,which contained all the genetic variance from Yongning populations detected by the 24 SSR loci.And the He and PIC of the nucleus germplasm were 0.595 8 and 0.586 3,respectively.This nucleus germplasm could basically be a representative for the genetic diversity of the Yongning common wild Oryza rufipogon Griff.populations.[Conclusion] There existed abundant genetic diversity in Yongning common wild Oryza rufipogon Griff.Populations.And it was suggested that the priority should be given to in-situ conservation and further utilization of genetic diversity of the wild Oryza rufipogon Griff.populations in this region.
【Fund】: 广西科技创新能力与条件建设资助项目(桂科能05112001-1A1 桂科能0630006-5B 桂科能0630006-5F)
【CateGory Index】: S511.9
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