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《Journal of Anhui Agricultural Sciences》 2008-11
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Establishment of RAPD Identification Method for Shigella

GUO Ai-ling et al(College of Food Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070)  
[Objective] The research aimed to provide the theoretical basis for tracking the epidemic origin of Shigella food-borne diseases and its disease control.[Method] Five random primers were used to make RAPD amplification on 4 different sources of Shigella.And the clustering analysis was made on the obtained amplification patterns.[Result] Among 5 random primers,primers S3 and S5 showed better polymorphism.In the amplification patterns by using primer S5,there were common bands including one band with about 600 bp and one band with about 1 000 bp in each bacteria of Shigella.When this primer was used to amplify other genera bacteria,the amplified bands was few or lack.This indicated that primer S5 was specific for bacteria of Shigella.According to the clustering analysis by using primer S5,8 strains of Shigella could be divided into 4 groups.Except for S.Sonnei,the similarity coefficients among 6 strains of 3 genera were all 1.00,which was completely accordant with the results of traditional serotype typing.[Conclusion] RAPD reaction by using primer S5 could be used in the fast detection of Shigella.
【CateGory Index】: R450
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