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《Journal of Anhui Agricultural Sciences》 2008-11
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Optimization of the Operation Rules for SSR Molecular Marker Technique in Maize

WANG Wei et al(College of Agronomy,Guizhou University,Guiyang,Guizhou 550025)  
[Objective] The research aimed to establish the operation rules for SSR marker technique in maize and provide basis for its application in the production.[Method] With 2 maize hybrids(Qiandan 16 and Xishan 99) and 4 maize inbred lines(Su 37,Jiao 51,Huang C and 4011) as tested materials,the genomic DNA was extracted from maize.And different DNA extraction methods,SSR reaction systems,PCR amplified procedures and silver staining methods were compared resp.[Result] The amplification effects of the DNA extracted by using SDS method or CTAB method were better.The optimum SSR reaction system in maize was as follows: 1U Taq enzyme,1×Bufer,60 ng template DNA,2.0 mmol/L Mg2+,0.15 mmol/L dNTPs,0.3 μmol/L primer.The optimum SSR amplification procedure was as follows: 93 ℃ for 1 min;30 cycles of 93 ℃ for 1 min,60 ℃ for 2 min,and 72 ℃ for 2 min;72 ℃ for 5 min.It was better to detect maize SSR marker by using 8% non-denaturing polyacrylamide gel electrophoresis or 6% denaturing polyacrylamide gel.[Conclusion] The genetic diversity of 51 maize hybrids in Guizhou were analyzed by using the optimized SSR marker detection system and most primers amplified clear bands.
【Fund】: 贵州省科技计划项目(黔科合NY字[2005]3021)资助
【CateGory Index】: S513
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