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《Journal of Anhui Agricultural Sciences》 2008-12
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Development of Colloidal Gold Rapid Diagnostic Stripe against Chicken Infectious Anaemia

SUN Su-ling(Anhui Science and Technology University,Fengyang,Anhui 233100)  
[Objective] The research aimed to lay the foundation for establishing the diagnostic method of chicken infectious anemia(CIA).[Method] After 15-week-old SPF chickens were immunized for 4 times,CIA serum antibodies were prepared and purified.The optimum coating concn.of antigen and the optimum dilution of serum were determined.And the protein content and titer of purified serum antibodies were studied.[Result] The optimum dilution of positive serum and negative serum was 1∶100 and the optimum coating concn.for coating antigen by virus was 5 μg/ml.The protein content of serum antibodies were 13.2 mg/ml and the titer of serum antibodies were 1∶12 800.[Conclusion] This research laid the foundation for preparing colloidal gold rapid diagnostic stripe against CIA
【Fund】: 安徽省教育厅资助重点项目(KJ2008A32ZC)
【CateGory Index】: S858.31
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