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《Journal of Anhui Agricultural Sciences》 2008-12
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Comparison of Determination Methods of Cynanchum otophyllum Schneid Pollen Activity

WANG Ding-kang et al(Department of Biology,Kunming College,Kunming,Yunnan 650031)  
[Objective] The study aimed to increase the fruit-set of Cynanchum otophyllum Schneid.[Method]Pollinaria were removed from the flowers by inserting an entomological pin.The membrane was removed under a dissecting microscope and the pollens were placed on the slid glass,the activities of C.otophyllum Schneid were determined by iodine-potassium iodide dyeing method(I2-KI),triphenyl tetrazolium chloride staining method(TTC),aceto carmine dyeing method and fluorescein diacetate method,the entire pollinaria were used in medium vitro germination method.[Result] The dyeing rate of I2-KI method,TTC method,aceto carmine dyeing method and FDA method were lower than 30.0%,but the pollen germination rate in medium vitro germination method reached to 97.6%.[Conclusion] Medium vitro germination method can be a effective method to determine the pollen activity of C.otophyllum Schneid.
【Fund】: 云南省应用基础研究计划项目(2007C262M);; 云南省教育厅科研基金重点项目(07Z10351)
【CateGory Index】: S567.239
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