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《Journal of Anhui Agricultural Sciences》 2008-13
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Study on the Extraction and Antibacterial Activity in Vitro of Flavonoids from Syzygium buxifolium Leaves

HUANG Xiao-kun et al(College of Educational Science,Quanzhou Normal University,Quanzhou,Fujian 362000)  
[Objective] The aim of the study was to discuss the feasibility of using flavoniods from Syzygium buxifolium leaves as botanical pesticide.[Method] The flavoniods were extracted and purified by the method of microwave treatment,alkali-extraction and acid-precipitation from S.buxifolium leaves.Its antibacterial activities on 7 bacterial species were determined by cylinder-plate method and their corresponding minimum inhibition concn.(MIC) were measured.[Result] The yield rate of crude flavonoids powder was 15.72%,with purity of 90.65%.The antibacterial circle diameters of flavoniods from S.buxifolium leaves at 100 g/L on Sarcina lutea,Staphylococcus aureus,Erwinia carotorora and Proteus vulgaris were bigger than 15 mm,especially on S.lutea and S.aureus,being up to 31.3 and 22.5 mm.The MIC of S.lutea was smallest(25 g/L),that of S.aureus and E.carotorora were secondary(50 g/L) and that of the other bacteria were 100 g/L.[Conclusion] Extraction and purification of flavonoids from S.buxifolium leaves by the method of microwave treatment,alkali-extraction and acid-precipitation, adsorption and elution by polyamide was simple,convenient and feasible.The flavonoids from S.buxifolium leaves had different antibacterial activities on different bacterial species.
【Fund】: 福建省教育厅科技项目(JA05318)
【CateGory Index】: S482.2
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