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《Journal of Anhui Agricultural Sciences》 2008-13
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Study on the Properties of Polyphenoloxidase from Coprinus comatus and Its Inhibitors

SHEN Li et al (Department.of Chemistry,Hebei Normal University of Science and Technology,Changli,Hebei 066600)  
[Objective] The aim of the research was to provide the theoretical basis for the fresh-keeping of Coprinus comatus.[Method] The crude enzyme liquid of polyphenoloxidase(PPO)was extracted from fresh C.comatus to study the effects of pH and temperature on PPO activity in C.comatus and analyze its thermal stability And the inhibitions of 3 kinds of inhibitors(sodium sulfite,ascorbic acid and citric acid) on PPO activity in C.comatus were discussed.[Result] In strong acidic and strong alkaline circumstances,PPO lost its activity.The enzyme activity of PPO showed a decreasing trend with the prolonging of heat-preservation time,which indicated that PPO was a kind of thermal-susceptible enzyme.Three kinds of inhibitors all had inhition on the activity of PPO from C.comatus.Among them,the inhibition of NaHSO3 was strongest,followed by ascorbic acid and citric acid.[Conclusion] In the processing course of C.comatus,adding NaHSO3 could effectively decrease PPO activity and prevent the occurrence of browning.
【CateGory Index】: TS201.1
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