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《Journal of Anhui Agricultural Sciences》 2008-14
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Study on in vitro Rapid Propagation of Lilium sulphureum

ZHANG Wen-e et al(Agricultural College of Guizhou University,Guiyang Guizhou 550025)  
[Objective] The research aimed to acquire the rapid propagation of Lilium sulphureum.[Method] taking MS media as basic media,the major factors which affected inducement of adventitious buds,multiplication of bulbs and radication of adventitious buds were studied.[Result] The hormone concentration,the diameter and the treating mode of bulbils and the inoculation time all effected on the induction of adventitious buds in first culture of Lilium sulphureum.It was the best to the induction of adventitious buds that the explants whose diameter was bigger than 1 cm,were taken in the last ten-day of August,after shucking off the outer squamas,and the bulbils were inoculated on the 1/2 MS+0.1mg/L IBA.The media types and second culture period all affected the multiplication of Lilium sulphureum.It had the best effect on multiplication to take 1/2 MS+0.1mg/L KT +0.1mg/L IBA as multiplication media and second culture every 40 d.In this way,the callus induction rate was 97.3%,the percentage of bulb induction was 100%,and the adventitious buds grew vigorous.In the 1/2 MS+0.1 mg/L IBA media,the radication was well.According the process of domestication in culture room and outdoors,then transplanting the seedlings,the transplant survival percentage of seedlings was more than 98%.[Conclusion] The domestication and transplanting of seedlings were carried out.This technical system can provide reference to the conservation and use of wild Lilium sulphureum resouces in Guizhou province.
【Fund】: 贵州省自然资金项目(黔科合J字[2007]2055号);; 贵州大学人才科研项目(贵大人基合字2006-700971301 贵大SRT字(20)号)
【CateGory Index】: S682.29
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