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《Journal of Anhui Agricultural Sciences》 2008-14
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Effects of Long-term Application of Manure on Bacteria Resistance Level to Tetracycline in Farmland Soil

WANG Yong et al(College of Life Science,Nanjing Normal University,Nanjing,Jiangsu 210046)  
[Objective] The purpose of the research was to investigate the environment risk of spreading the livestock and poultry manure produced by intensive culture into farmland directly.[Method] With the soil applied chemical fertilizer for a long time as CK,the effects of long-term application of fresh chicken and pig manures on the tetracycline resistance bacteria in soil was studied through fixed-plot test.[Result] The amount of tetracycline resistance bacteria and its proportion accounting for the total amount of cultured bacteria in fresh chicken and pig manure were greatly higher than that in the basic soil sample before manure application.And after long-term application of these manure the amount and its proportion accounting for the total amount of cultured bacteria in soil were significantly higher than that in CK and this increase trended to vertical increment with the soil depth of 0-20 cm.[Conclusion] Long-term application of manure could lead the resistance level of the antibiotic antibiotics to increase greatly,which caused a potential risk to environment.
【Fund】: 国家自然科学基金项目(20607024);; 国家“十一五”科技支撑计划资助项目(2006BAD10B05)
【CateGory Index】: S154.3
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