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《Journal of Anhui Agricultural Sciences》 2008-15
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Analysis on Genetic Characters of Arabidopsis thaliana Mutant Insensitive to Reactive Oxygen

HE Jin-huan et al(Department of Bioengineering,Zhengzhou College of Animal Husbandry and Engineering,Zhengzhou,Henan 450011)  
[Objective] The study aimed to explore the genetic characters of Arabidopsis thaliana mutant insensitive to reactive oxygen.[Method] The seeds of A.thaliana after being sterilized were cultivated in the MS solid culture medium.After cultivated for 5~6 d,the seedlings were transplanted in vermiculite and cultivated again.The hybridization was made with the wild type as male and the mutant as female in flowering stage.The seeds of F1 were planted in MS culture medium.After growing for 5~6 d the seedlings were treated with 10-4 mol/L H2O2,the resistance of seedlings to H2O2 was detected by the root bending method and the genetic characters of the mutants were analyzed.[Result] The total seedlings of F1 were nonresistant to H2O2,i.e.the growth of roots was restrained.184 plants were nonresistant to H2O2,while 59 plants were resistive to H2O2 among 243 plant in F2.The separation ratio of nonresistant plant to resistant plants was 3∶1.[Conclusion] There was a single recessive gene mutation happened in A.thaliana mutant insensitive to reactive oxygen.
【CateGory Index】: Q943
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