Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Agricultural Sciences》 2008-15
Add to Favorite Get Latest Update

Study on the Separation and Identification of Polyphenol in Lotus Root by Thin-layer Chromatography

LIU Ji-hong et al(College of Sciences,Huazhong Agricultural University,Wuhan,Hubei 430070)  
[Objective] The research aimed to lay the foundation for separating and identifying polyphenol from lotus root.[Method] With silica gel G60 as stationary phase,with toluene-ethyl acetate-icy acetic acid as mobile phase,with catechin as control substance,with FeCl3-K3Fe(CN)6 as chromogenic reagent,phenolic compounds were separated and identified from ester-phase components of polyphenol in lotus root by using thin-layer chromatography(TLC).And the effects of different developing solvent on the separation of components were analyzed.[Result] Rf values between the 2nd spot evolved by ester-phase of polyphenol in lotus root and the spot of standard reagent catechin was the same,which preliminarily confirmed that there was catechin in the ester-phase of polyphenol in lotus root.Better separation effects all could be obtained by using 6 kinds of developing reagents in TLC and their Rf was in the range of 0.49~0.61,which accorded the qualitative demands of Rf in TLC.[Conclusion] TLC was highly efficient,sensitive and rapid,which provided better detection method for preliminarily identifying the compounds of catechin in lotus root.
【CateGory Index】: S645.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 4 Hits
1 YAN Shou -lei,WANG Qing -zhang,PENG Guang -hua (Food Science Department,Huazhong Agricultural University,Wuhan 430073,China);Exploration of Boiled Lotus Root Slices Processing Technology[J];Storage and Process;2002-03
2 WANG Qing-zhang~*, PEN Guang-hua , JIN You, LI Jie , YAN Shou-lei (Department of Food Science and Technology,Huazhong Agricultural University, Wuhan 430070);Extraction of Polyphenol from Lotus Roots and Its Enzymatic Browning Substrate[J];Journal of Analytical Science;2004-01
3 YAN Shou-lei1,2 ,WANG Qing-zhang1, PENG Guang-hua1 ,GAO Zhi-xian2 (1.Food Science and Technology College, Huazhong Agricultural University, Wuhan 430070,Hunan, China;2.Institute of Hygienic and Environmental Medicine Academy of Military Medical Sciences, Tianjin 300050,China);STUDY ON THE EXTRACTION OF LOTUS ROOT POLYPHENOL[J];Food Research and Development;2006-04
4 Yu Jiandong, Wang Gangli, Gao Tianbing, Tian Jingai National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, P. R. China;Study on Chemical Composition of Catechu ( I )——Distinction Between Catechin and Epicatechin by TLC[J];China Pharmacist;2000-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHOU Zhen-bing et al (Guangxi College of Agricultural Vocation and Technique,Nanning,Guangxi 530007);Extraction,Separation and Purification of Polyphenols from Sargassum and Its Antimicrobial Activity Test[J];Journal of Anhui Agricultural Sciences;2009-17
2 SHAO Wei-liang,HU Tian-xi,HANG Xiao-min,DING Wei-qi,YU Chen-qing(Shanghai Jiaoda Onlly Life Sci-tech Development Co.Ltd,Shanghai 200030);Study on the quality index of the lipid tea polyphenols based on their effects of antioxidation and anti-lipid peroxidation in different esterification degree[J];Anhui Medical and Pharmaceutical Journal;2006-12
3 LU Cong-cong1,SHAO Wei-liang2,HANG Xiao-min2,ZHANG Wei2,YAO Xiao-min1(1.School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240;2.Shanghai Jiaoda Onlly Life Sci-tech Development Co.Ltd,Shanghai 200233);Two kinds of chemical modification on tea polyphenols and study on the antioxidant activity of the lipid-soluble tea polyphenols[J];Anhui Medical and Pharmaceutical Journal;2008-03
4 XU Jin-yun1,WANG Qing-zhang 1,2,HE Jian-jun2,ZHOU Ming-quan2,HU Zhong-li 2 (1.College of Food Science,Huazhong Agricultural University,Wuhan 430070; 2.Lotus Research Centre,Wuhan University,Wuhan 430072, China);Study on Storage and Physiology of Enlarged Rhizome of Lotus[J];Amino Acids & Biotic Resources;2003-02
5 DIAO Ying 1, HANG Yan-chuang 1, HE Jian-jun 2, WANG Qing-zhang 2, HU Zhong-li 1,2 , ZHOU Ming-quan 2 (1.Key Laboratory of MOE for Plant Developmental Biology,Wuhan University; 2.Lotus Research Center,Wuhan University,Wuhan 430072, China);Advances of Study on Lotus[J];Amino Acids & Biotic Resources;2004-01
6 DIAO Ying ( Key Laboratory of MOE for Developmental Biology, Wuhan University; Biology Department, West Chongqing University,Yongchuan Chongqing 402168 Lotus Research Center, Wuhan University, Wuhan 430072, China);A Study of the FISH Protocol on Lotus Chromosomes[J];Amino Acids & Biotic Resources;2004-02
7 DIAO Ying~(1,2),SHE Chao-wen~(3*),HU Zhong-li~1(1 Key Laboratory of MOE for Plant Development Biology,College of Life Science,Wuhan University,Wuhan 430072;2 Department of life science,Chongqing University of Arts and Sciences,Chongqing 400068;3 Lotus Center,Wuhan University,Wuhan 430072,China);Karyotype Analysis for Zygotene Chromosome of Nelumbo nucifera[J];Amino Acids & Biotic Resources;2006-03
8 KONG De-zheng;JIN Dan-dan;HE Song-lin;YANG Qiu-sheng;LIU Yi-ping.Forestry and Horticulture College,Henan Agriculture University,Zhengzhou,450002,P.R.China.;Anatomy and changes in endogenous hormones during flower bud differentiation in Nelumbo nucifera.[J];Journal of Beijing Forestry University;2009-03
9 DENG Yuan-xi, XU Hui, MA Long, REN Mao-sheng (Department of Food and Bioengineering, Bengbu College, Bengbu Anhui 233030, China);Study on the Processing Technique of Soft-packed of Sweet-scented Osmanthus Sticky Rice Sugar Lotus Root[J];Packaging and Food Machinery;2009-02
10 Zhao Donghai, Wang Yun, Zhang Jianping;Studies on the Biochemical Conditions of Browning Promoted by Enzyme in Fruit and Vegetable[J];Journal of Changjiang Vegetables;2005-07
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YIN Shuai-wen et al (College of Life Science,Jinggangshan University,Jian,Jiangxi 343009);Primary Study on the Antifungal Activity of Several Ferns[J];Journal of Anhui Agricultural Sciences;2008-04
2 LI Rong-qin et al(Tibet Agriculture Aollege,Linzhi,Tibet 860000);Study on Usnea Acid Content in the Usnea Longissima[J];Journal of Anhui Agricultural Sciences;2008-06
3 Wang Zhenyu, Yang Chunyu, Jin Zhongyue(Northeast Forestry University, Harbin 150040, P. R. China);The Technology of Abstracting Aloe Gelatin by Ultrasonic[J];Journal of Northeast Forestry University;2002-04
4 ZHOU Ben-jie, ZHANG Zhong-yi, SHI Yong, GU Wei-xin, ZHANG Shou-yaoDepartment of Pharmacy, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China;Analysis with gas chromatography and mass spectrography of volatile components of Ligus-ticum wallichii Franch extracted by CO_2 supercritical fluid extraction in combination with molecular distillation[J];Journal of First Military Medical University;2002-07
5 GUAN Ming qiang et al. (Yunnan Agriculture University Animal Nutrition and Feed Laboratory,Kunming 650201)$$$$;Bioactkivitity additive-Saponin[J];Animal Science Abroad;2001-05
6 SONG Wei-wei, JIAO Shi-rong, ZHOU Jia, YE Chun-yan (School of Bioengineering, Xihua University, Chengdu 610039, China);Microwaves Assisted Extraction of Polyphenol from Pomegranate Peel and its Antioxidant and Antimicrobial Activities[J];Modern Food Science and Technology;2008-01
7 Lin Jie; Wu Jinzhu; Zhu Xingui ;Mei Qianbiao; Guo Zhimin(Dept. of Food Science, South China Agric. Univ. Guangzhou, 510642);Studies on Anti-Microbial Activities of Shaddock Peel Extract[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1999-03
8 LIU Guang-xi Zhuzhou City Hospital of Traditional Chinese Medicine,Zhuzhou,Hunan,China,412000;Study Overview on the Separation and Purification Technique for Effective Component of Chinese Herbs[J];Guiding Journal of Traditional Chinese Medicine and Pharmacy;2008-07
9 GU Hong-mei,ZHANG Xin-kun,JIANG Xiao-ping (Key Lab.of Leather Chemistry and Engineering of Ministry of Education, Sichuan University,Chengdu 610065,China);Ultrasonic method for extracting anthocyanins from purple sweet potato[J];Chemical Research and Application;2004-03
10 ZHANG Hui-li, SUN Yin-shi, ZHENG Yi-nan (College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118,China);Study on Isolation Method of Ginsenoside Re by Sephadex LH-20[J];Journal of Jilin Agricultural University;2005-03
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 Wang Qingzhang; Shi Guorong; We Chengyin;Influence of Several Chemicals to Control Browning on Storage of Husking Potato Segment[J];JOURNAL OF CHANGJIANG VEGETABLES;1998-11
2 Lu Jiajiong, Li Qingtao, Guo hairong,Liu Manping (Guangxi University, Nanning 530004);Study on Factors of Effecting Maillard Reaction in Juice[J];Sugarcane and Canesugar;2000-02
3 Wang Sanhu; et al.(Food Processing Department);Studies on polyphenol Oxidase Main Chracters in Lotus Root and Prevention of Discoloration[J];JOURNAL OF HENAN VOCATION-TECHNICAL TEACHERS COLLEGE;1994-01
4 Sun Wei 1) Ding Baoliang 2) Yu Guanjun 3) Tao Yiwei 3) Shi Yimin 3) ( 1) Shanghai Light Industry College,Shanghai 200433; 2) Nantong Teachers College,Jiangsu Nantong 226007; 3) S;STUDIES ON VEGETABLE PHYSIOLOGY AND QUALITY PRESERVATION IN PRODUCTION OF SEMI PROCESSED CUT VEGETABLES(Ⅰ)[J];ACTA AGRICUL TURAE SHANGHAI;1999-03
5 Hu Jun(Department of Food Science Zhejiang Agriculture University, Hangzhou);The Characters of Polyphenol Oxidase in Lotus Root and Prevention of Discoloration of Lotus Root[J];Food and Fermentation Industries;1989-03
6 Ji Rong, Zhong Kai, Li Yepeng, Li Yanjun, et al. Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021,China;Effects of tea polyphenols on mice survival rate and white blood cell count after radiation[J];Journal of Hygiene Research;2002-05
7 WANG Chuan xian, ZHONG Jin yi (Institute of Nutrition, Medical College of Qingdao University, Qingdao 266021, China);THE SCAVENGING EFFECTS OF GRAPE PROCYANIDINS ON ACTIVE OXYGEN FREE RADICALS[J];Acta Nutrimenta Sinica;2001-02
8 CHEN Zhi hua, LI Zhan jing (Wuxi Sci. Res.& Design Inst of the State Administration of Grain Reserve);Property Experiment of Pulverous Liposoluble Green Tea Polyphenols in Salad Oils[J];CHINA OILS AND FATS;2000-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved