Comparison on Bioactivities of Solanesol Extracted from Tobacco Leaves by Different Methods
GUO Yi et al(Key Laboratory of Water and Sediment Sciences,Ministry of Education,Department of Environmental Engineering,Peking University,Beijing 100871)
[Objective] The aim was to select suitable method for extracting solanesol from tobacco leaves.[Method] DPPH method and agar plate diffusion were used to investigate the antioxidant and antibacterial activities of solanesol extracted from tobacco leaves by 3 methods.[Result] The rates of semi-scavenging(IC50) of solanesol extracted by soxhlet,ultrasonic and supercritical extractions from saponated tobacco leaf powder on DPPH were 56.43,54.77 and 49.48 mg/L resp.,that of solanesol extracted by soxhlet and ultrasonic extractions from non saponated tobacco leaves on DPPH were 47.22 and 44.45 mg/L resp.and that of Vc(CK) was 13.89 mg/L.The curve of DPPH scavenge rate of mixture of solanesol and Vc with equal volumes was between theirs.The antibacterial activities of solanesol extracted by different methods in order were supercritical extraction soxhlet extraction ultrasonic extraction.Saponification had negative effects on both antioxidant and antibacterial activities of extracts.[Conclusion] The bioactivities of solanesol extracted from tobacco leaves by different methods in order were supercritical extraction soxhlet extraction ultrasonic extraction.