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《Journal of Anhui Agricultural Sciences》 2008-16
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Establishment of PCR Method for Detecting Porcine Circovirus 2

HANG Bo-lin et al (College of Animal Science,Henan Institute of Science and Technology,Xinxiang,Henan 453003)  
[Objective] The aim of the research was to provide reference for rapid diagnosis of porcine circovirus disease.[Method] According to the nucleotide sequence of PCV-2 on GenBank website,one pair of primers were designed and synthesized.And PCR method for diagnosing the local isolates of PCV2 was established.[Result] The optimum concn.of each substance in PCR reaction were as follows: dNTP 220 pmol/ml,Taq enzyme 0.1 U/μl,primers 20 pmol/μl. DNA was extracted from diseased materials with the positive PCV-2 to make PCR amplification under the optimized conditions,and the expected specific bands with the size of 494 bp was obtained.PCV-2,PRRSV,HCV,SIV and PPV were detected by this PCR method,PCV-2 was positive and PRRSV,HCV,SIV and PPV were negative,without cross reaction.This indicated that this PCR method had good specificity to PCV-2.1.25 template DNA could be detected by this PCR method which had higher sensitivity.[Conclusion] It was feasible to detect PCV-2 by using this PCR method.
【Fund】: 河南科技学院大学生科技创新基金
【CateGory Index】: S854.43
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 CUI Ke,LIANG Yuan-xiang,LI Bu-yong,ZHOU Qiu-ping (The Center for Animal Epidemic Disease Diagnostics of Hainan Province, Haikou 570203,China);An Advance in Diagnostic Techniques of Porcine Circovirus Disease[J];Natural Science Journal of Hainan University;2006-04
2 WANG Hai-hua,LI Yan-ling,WANG De-gang,ZHANG Gui-yun(Zhengzhou College of Animal Husbandry Engineering,Henan 450011,China);Study on Porcine Circovirus[J];Acta Ecologiae Animalis Domastici;2006-04
3 PAN Rao-lin,SHAN Song-hua,LIU Hong-yun,LI Chun-hua,CHEN Xiao-qing,ZHAO Ben-jin,PENG Li-ying,LIU Sheng,WEN Yi,ZHANG Hua-di,XU Ping,QIAN Zhong-hui(Shanghai Jiamu Biological Products Co.,Ltd,Shanghai 201106,China);Isolation and identification of porcine circovirus type 2[J];Acta Agriculturae Shanghai;2006-03
4 LANG Hong wu 1, ZHANG Guang chuan 1, WU Fa quan 2, ZHANG Chuang gui 2 (1. National Institute for Control of Veterinary Bioproducts and Pharmaceuticals,Beijing, 100081; 2. Pigs Feeding and Breeding Center of Beijing,Beijing, 100081);Detection of Serum Antibody against Postweaning Multisystemic Wasting Syndrome in Pigs[J];CHINESE JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY;2000-03
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 JIN Yu-fen (College of Food Science and Biotechnology,Anyang Institute of Technology,Anyang,Henan 455000);Establishment and Application of Nested PCR Assay for Testing PCV2[J];Journal of Anhui Agricultural Sciences;2007-07
2 HAO Gui-zeng et al (Department of Animal Husbandry and Veterinary,Anyang College of Technology,Anyang,Henan 455000);Research Progess of Molecule Biology of Porcine Circovirus[J];Journal of Anhui Agricultural Sciences;2007-13
3 YI Dao-sheng et al(Huo Ying-dong College of Bioengineering,Shaoguan College,Shaoguan,Guangdong 512005);Multiplex PCR Detection and Typing of Porcine Circovirus[J];Journal of Anhui Agricultural Sciences;2007-32
4 BAI Jing et al (Department of Bioengineering,Zhengzhou College of Animal Husbandry Engineering,Zhengzhou,Henan 450011);Isolation and Identification of Porcine Circovirus Henan Strain[J];Journal of Anhui Agricultural Sciences;2008-13
5 SHI Dong-mei et al (Zhengzhou College of Animal Husbandry Engineering,Zhengzhou,Henan 450011);Serology Detection Report of Porcine Contagious Pleuropneumonia in Clinical Infection[J];Journal of Anhui Agricultural Sciences;2008-25
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Cao Shengbo Chen Huanchun Xiao Shaobo He Qigai Xu Yindi Liu Junfa (Huazhong Agricultural University, Wuhan 430070,China);The Establishment And Application of PCR to Diagnosis Porcine Circovirus Type 2[J];Journal of Huazhong Agricultural;2001-01
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