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《Journal of Anhui Agricultural Sciences》 2008-16
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Establishment of PCR Method for Detecting Porcine Circovirus 2

HANG Bo-lin et al (College of Animal Science,Henan Institute of Science and Technology,Xinxiang,Henan 453003)  
[Objective] The aim of the research was to provide reference for rapid diagnosis of porcine circovirus disease.[Method] According to the nucleotide sequence of PCV-2 on GenBank website,one pair of primers were designed and synthesized.And PCR method for diagnosing the local isolates of PCV2 was established.[Result] The optimum concn.of each substance in PCR reaction were as follows: dNTP 220 pmol/ml,Taq enzyme 0.1 U/μl,primers 20 pmol/μl. DNA was extracted from diseased materials with the positive PCV-2 to make PCR amplification under the optimized conditions,and the expected specific bands with the size of 494 bp was obtained.PCV-2,PRRSV,HCV,SIV and PPV were detected by this PCR method,PCV-2 was positive and PRRSV,HCV,SIV and PPV were negative,without cross reaction.This indicated that this PCR method had good specificity to PCV-2.1.25 template DNA could be detected by this PCR method which had higher sensitivity.[Conclusion] It was feasible to detect PCV-2 by using this PCR method.
【Fund】: 河南科技学院大学生科技创新基金
【CateGory Index】: S854.43
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