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《Journal of Anhui Agricultural Sciences》 2008-17
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Cloning and Sequence Analysis of Biological Clock Gene Bmtim2 in Bombyx mori L.

ZHAO Hao-qin et al(Jiangsu University of Science and Technology,Zhenjiang,Jiangsu 212003 )  
[Objective] The research aimed to provide basis for further research on the functions of biological clock gene in Bombyx mori L.[Method] Total RNA was extracted from unfertilized egg of silkworm variety p50.Bmtim2 cDNA sequence of the biological clock gene was cloned by using rapid amplication of cDNA ends(RACE) technology.Finally the expression of this gene in different growth stages of B.mori was preliminarily studied by using Real-time quantitative PCR.[Result] cDNA sequence of the biological clock gene timeless2(Bmtim2)in B.mori with the length of 1 867 bp which included complete 3' untranslated region was obtained through RACE amplification.Through derivating,the protein sequence with 584 amino acid residues that was coded by this gene sequence was obtained.The protein sequence had C-terminal conservative region of TIM protein and it had closely-related evolution relationship with TIM 2 gene of Drosophila melanogaster and Culex pipiens.Bmtim 2 gene was expressed at each time point of the life cycle in B.mori.[Conclusion] These results had an important reference value for studying the time-space expression differences of Bmtim2 gene in the growth process of B.mori and its relationship with the circadian rhythm.
【Fund】: 国家高技术研究发展计划“863计划”(2006AA10A119);; 江苏省自然科学基金前期预研重大项目(BK2004206)
【CateGory Index】: S881
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