Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Agricultural Sciences》 2008-17
Add to Favorite Get Latest Update

Cloning and Sequence Analysis of Biological Clock Gene Bmtim2 in Bombyx mori L.

ZHAO Hao-qin et al(Jiangsu University of Science and Technology,Zhenjiang,Jiangsu 212003 )  
[Objective] The research aimed to provide basis for further research on the functions of biological clock gene in Bombyx mori L.[Method] Total RNA was extracted from unfertilized egg of silkworm variety p50.Bmtim2 cDNA sequence of the biological clock gene was cloned by using rapid amplication of cDNA ends(RACE) technology.Finally the expression of this gene in different growth stages of B.mori was preliminarily studied by using Real-time quantitative PCR.[Result] cDNA sequence of the biological clock gene timeless2(Bmtim2)in B.mori with the length of 1 867 bp which included complete 3' untranslated region was obtained through RACE amplification.Through derivating,the protein sequence with 584 amino acid residues that was coded by this gene sequence was obtained.The protein sequence had C-terminal conservative region of TIM protein and it had closely-related evolution relationship with TIM 2 gene of Drosophila melanogaster and Culex pipiens.Bmtim 2 gene was expressed at each time point of the life cycle in B.mori.[Conclusion] These results had an important reference value for studying the time-space expression differences of Bmtim2 gene in the growth process of B.mori and its relationship with the circadian rhythm.
【Fund】: 国家高技术研究发展计划“863计划”(2006AA10A119);; 江苏省自然科学基金前期预研重大项目(BK2004206)
【CateGory Index】: S881
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 LIU Zong Min ZHANG Ya Ping ① (Laboratory of Cellular and Molecular Evolution,Kunming Institute of Zoology,the Chinese Academy of Sciences,Kunming 650223,China);Timeless Gene and Biological Clock Genes[J];Zoological Research;2001-06
2 Han Zhiyong Wang Xinqi Shen Gezhi (Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106);CLONING OF FOREIGN GENE'S FLANKING SEQUENCES IN TRANSGENIC RICE BY INVERSE PCR[J];Acta Agriculturae Shanghai;2001-02
3 HU Jian-Guan; ZHAO Xiang-Shan; LIU Jun; YUAN Zi-Qiang; YANG Jin-Shui (Institute of Genetics, Fudan University, Shanghai 200433);Isolation and Characterization of a cDNA Encoding Maize Cytosolic Malate Dehydrogenase[J];ACTA BOTANICA SINICA;1999-01
Chinese Journal Full-text Database 10 Hits
1 SONG Yan ZHU Xiao-Su XU Li XU Shi-Qing(Department of Applied Biology,School of Basic Medicine & Biological Science,Soochow University,Suzhou Jiangsu 215123,China);The Molecular Mechanism of Inset Circadian Clock Entrained by Light and Temperature[J];Science of Sericulture;2009-02
2 ZHAO Wei,XU Wei-rong,XU Yan,HE Ming-yang,WANG Yue-jin(College of Horticulture,Northwest A & F University,Key Laboratory of Horticulture Plant Germplasm and Genetic Improvement of China Agricultural Ministry,Yangling,Shaanxi 712100,China);Cloning Full Length cDNA of Chinese Wild Vitis Novel Gene and Sequence Analysis[J];Acta Botanica Boreali-Occidentalia Sinica;2007-05
3 JIANG Rui hua, LIN Chang fa, SHEN Guo an, QIAN Min, WANG Dong, DU Xi ling, HUANG Ji, YANG Jin shui (Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433,China);Cloning of the Rice Cytoplasmic Malic Dehydrogenase Gene ( Rc MDH ) and Its Expression in E.coli[J];Journal of Fudan University;2002-01
4 YAN Jing-wan~(1,2), LIN Lin~1, WANG Feng~2(1. College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350007, China; 2. Key Laboratory of Agriculture Genetic Engineering, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China);Amplified methods for acquiring gene flanking sequence's information[J];Fujian Journal of Agricultural Sciences;2005-S1
5 Yin Hui Li Dan Zhang Yi Li Qiuli* College of Life Sciences, Liaoning Normal University, Dalian, 116029;Cloning Methods of Plant Gene Promoters and Their Applications[J];Molecular Plant Breeding;2006-S1
6 CHEN Zhao-gui(Department of Science, Huizhou University, Huizhou 516015 China);Progress in Transposon and its Use in Studying Functional Genomics of Rice[J];Journal of Huizhou University;2003-06
7 GUO Yu qi et al . (Dept. of Agronomy,Luoyang Agricultural College,Luoyang,471003);Gene Expression and the Mechanisms of Heterosis[J];Journal of Henan Vocation-technical Teachers College;2001-02
8 CHI Yan1,2,ZHOU Dong-po1,PING Wen-xiang1,LI Shan-shan1,ZHU Jing1(1.Key Laboratory of Microbe,Heilongjiang University,Harbin 150080,China;2.Key Laboratory of Bio-organic Chemistry,Dalian University,Dalian 116622,China);Obtaining FSTs of Nodulisporium sylviforme fusant by TAIL-PCR techniques[J];Journal of Liaoning Normal University(Natural Science Edition);2007-01
9 ZHANG Zhuan XUE Wei WANG Xiang-Kun SUN Chuan-Qing**(Department of Plant Genetics and Breeding,State Key Laboratory for Agrobiotechnology,Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture,Beijing Key Laboratory of Crop Genomics and Genetic Improvement,China Agricultural University,Beijing 100094,China);Studies on the Relationship between Heterosis and Gene Differential Expression Using Introgression Lines of Rice(Oryza sativa L.)[J];Journal of Agricultural Biotechnology;2005-06
10 ZHANG Fu-Li,LEI Shao-Rong,LIU Yong Analysis and Determination Center,Sichuan Academy of Agricultural Sciences;Ministry of Agriculture Environmental Safety Supervision and Inspection Center for Genetically Modified Plants in Chengdu;Chengdu 610066,China;Detection Methods for Transgenic Crops and its Processed Goods[J];Letters in Biotechnology;2009-05
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 ZHENG Xiang zhong ①② ZHANG Ya ping ① ZHU Ding liang ② GENG Zhen cheng ② (① Lab of Cellular and Molecular Evolution,Kunming Institute of Zoology, the Chinese Academy of Sciences,Kunming 650223) (② Institute of Genetics,Fuda;THE MOLECULAR BIOLOGY OF THE BIOLOGICAL CLOCK GENE, period[J];ZOOLOGICAL RESEARCH;1998-06
2 Shen Gezhi; Zhang Jianjun; Yin Liqin; Wang Xinqi; Chen Quanqin; Fan Kunhua (Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences, Shanghai 201106);Agrobacterium-MEDIATED Ds TRANSPOSON TRANSFORMATION IN RICE (Oryza sativa L.)[J];ACTA AGRICUL TURAE SHANGHAI;1998-04
3 WANG Huai; CHEN Zheng-Hua(Institute of Genetics, Chinese Academy of Science, Beijing 100101);Nuclear matrix attachment regions and transgene expression in plants[J];CHINESE BULLETIN OF LIFE SCIENCES;1999-02
4 HUA Zhi-Hua; HUANG Da-Nian(China National Rice Research Insttitute, Hanzhou 310006);Genetic Mode of Exogenes in Transgenic Plants[J];ACTA BOTANICA SINICA;1999-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved