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《Journal of Anhui Agricultural Sciences》 2008-18
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Establishment and Optimization of SRAP Amplification System in Bitter Melon(Momordica charantial L.)

GUO Da-long et al(College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003)  
[Objective] Molecular marker provided a more reliable and efficient method to evaluate the genetic diversity for bitter melon.[Method] The optimum SRAP-PCR reaction system in bitter melon(Momordica charantial L.) was established with orthogonal design on four levels of five factors(Mg2+,dNTPs,primer,Taq polymerase and DNA template).[Result] In a total volume of 20 μl SRAP-PCR system,the optimum SRAP-PCR reaction system contained 1× buffer,1.0 ng/μl DNA template,1.5 mmol/L Mg2+,0.3 mmol/L dNTPs,0.5 μmol/L primer and 0.075 U/μl Taq polymerase.[Conclusion] The optimized SRAP-PCR reaction system would provide the basis for the analysis of diversity,map construction and gene localization of important traits of bitter melon with SRAP markers.
【Fund】: 河南科技大学人才启动基金(09001163);; 河南科技大学校基金(2007QN014)
【CateGory Index】: S642.5
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