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《Journal of Anhui Agricultural Sciences》 2008-19
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Study on the Transformation Method of Mediating Exogenous Gene into Cotton Epicotyl by Agrobacterium

YANG Li-heng et al (College of Agronomy,Xinjiang Agricultural University,Urumuqi,Xinjiang 830052)  
[Objective] The aim was to introduce a new method of obtaining transgenic cotton plants.[Method] With the competent germinating epicotyl of cotton as receptor in exogenous gene transformation,NPTII(neomycin phosphotransferase II) gene carrying selection marker and GFP(green fluorescent protein) reporter gene were mediated into cotton by agrobacterium-mediated transformation.The preculture time,bacterial-immersing time,co-culture time and antibiotics concn.,etc of using agrobacterium-mediated transformation in the transformation of competent germinating epicotyl of cotton were studied.[Result] Based on directly regenerated plant of in vitro plant of Xinjiang cotton,the cotton epicotyl treated by pre-culture for 3-5 d was immersed in bacteria for 15 min and co-cultured for 2 d.Cephalothin at 1 000 mg/L was used to inhibit bacteria after 50 mg/L kan selection.And the combination of cytokinin with relatively high content and auxin with low content was used in its directly budding culture to obtain resistant seedlings.[Conclusion] Treating cotton epicotyl by agrobacterium transformation was a simply operative and genotype independent transformation method with good reproducibility.
【Fund】: 新疆农业科学院院长基金项目(2007Y09)资助
【CateGory Index】: S562
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