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《Journal of Anhui Agricultural Sciences》 2008-19
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Infection of Aeromonas veronii from Diseased Cyprinus carpio L.and the Biological Characteristics of Its Pathogen

QIN Guo-min et al (Key Laboratory of Oceanic Biotechnology of Jiangsu Province,Huaihai College of Technology,Lianyungang,Jiangsu 222005)  
[Objective] This study was to report the diseased situation of Cyprinus carpio L.and the main biological characteristic of its pathogen.[Method] The pathogenic bacteria from tissues of five diseased or dead C.carpio were examined and isolated,and the identification of phenotypic information of isolates was conducted,including the morphological characteristics,physiological and biochemical characteristics.The representative strains were selected to make the molecular identification of 16S rRNA gene,the artificial infection test and antibiotic sensitivity test.[Result] According to the morphological characteristics,physiological and biochemical characteristics of 2 isolates,combined with the results of 16S rRNA gene sequence and phylogenetic analyses it was found that 2 isolates were belonged to Aeromonas veronii and the sequenced length of 16S rRNA gene of the representative strain HC050630A-1 was 1 457 bp.The artificial infection experiment indicated that the strain HC050630A-1 had strong pathogenicity.The antibiotic sensitivity test using 37 antimicrobial agents showed that the strain HC050630A-1 were high sensitive to 16 agents including cephradine,were sensitive to 13 agents including cefazolin and were resistant to 8 agents including penicillin G.[Conclusion] This study provide reference for effective checkout and prevention etc.of the disease of C.carpio.
【Fund】: 淮海工学院引进人才科研启动基金资助(KQ07106)
【CateGory Index】: S943
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