Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Agricultural Sciences》 2008-20
Add to Favorite Get Latest Update

Screening of Surface Sterilization Methods in the Tissue Culture of Zoysia matrella

WANG Dong et al (Chongqing Key Laboratory of Forage and Herbivore,College of Animal Science and Technology of Southwest University,Research Center of Herbivore Engineering in Universities of Chongqing,Beibei,Chongqing 400716)  
[Objective] The research aimed to lay the foundation for establishing the regeneration system of Zoysia matrelia.[Method] With stem with buds of Zoysia matrella as explants,the effects of such factors as 0.1%mercuric chloride,5%sodium hypochlorite,75%alcohol and pretreatment methods on the survival rate of explants were studied though the orthogonal test.And the optimum surface sterilization methods was screened out.[Result] The effects of each factor on the survival rate of explants orderly were 0.1%mercuric chloride 5%sodium hypochlorite 75%alcohol pretreatment methods and the effects of 0.1%mercuric chloride and 5%sodium hypochlorite reached at extremely significant level and at a significant level.The optimum sterilization method in the tissue culture of Zoysia matrella was as follows: flushing with 5 g detergent and water for 2 times, and sterilizing with 5%sodium hypochlorite(dropping 3 drops of 100%Tween 80) for 10 min under vibratory conditions and finally sterilizing with 0.1%mercuric chloride(dropping 3 drops of 100%Tween 80) for 10 min under vibratory conditions.[Conclusion] It could play the role of sterilization and bacteriostasis to pretreat explants with bactericide,antibiotic and so on or adding antibiotic to the medium.
【Fund】: 国家重点基础研究发展计划(2007CB108901)资助
【CateGory Index】: S688.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved