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《Journal of Anhui Agricultural Sciences》 2008-20
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Research on the Effects of Polysaccharides from Zhiqi Fungal Substance on CEF Proliferation and Resisting NDV Infection

ZHANG Dun-lin et al (College of Chemical Science and Engineering,Yunnan University,Kunming,Yunnan 650091)  
[Objective] The purpose was to confirm whether the polysaccharides from Zhiqi fungal substance were its effective components enhancing the immunity of animal.[Method] Ethanol at different concn.was used in the fractional precipitation of the water extracts from Zhiqi fungal substance and 3 polysaccharides(GAP30,GAP60 and GAP90) were obtained from Zhiqi fungal substance.Their effects on the proliferation of chicken embryo fibroblast(CEF) and resisting NDV infection were studied by neutral red dye absorption spectrometry.[Result] The yield of GAP30,GAP60 and GAP90 were 0.37%,0.70% and 1.04% resp.,showing the polysaccharides in Zhiqi fungal substance had been extracted completely by fractional precipitation with ethanol.When GAP30 and GAP60 were 1×10-1 and 1×10-2 mg/ml and when GAP90 was 1×10-3 mg/ml,they could promote CEF proliferation significantly.When GAP30 and GAP60 were 1×10-2 mg/ml and when GAP90 was 1×10-1 or 1×10-2 mg/ml,they could significantly resist NDV infection.[Conclusion] The polysaccharides in Zhiqi fungal substance could promote CEF proliferation and resist NDV infection to different degrees and the effect of GAP90 was best.
【Fund】: 南京晓庄学院院级科研项目青年专项(2007NXY28)
【CateGory Index】: S816.7
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