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《Journal of Anhui Agricultural Sciences》 2008-21
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Study on In Vitro Stem Culture and Rapid Propagation of Prunus cerasifera Ehrh.cv.Atropurpurea

LIU Chang-chun et al (Garden and Flower Engineering Research Center of Chongqing Colleges,Chongqing 400041)  
[Objective] The study aimed to lay the base for the commercial production,genetic transformation and variety-directed improvement of Prunus cerasifera Ehrh.cv.Atropurpurea.[Method] With the stems of P.cerasifera as the explants,6-BA and NAA with different concn.were added into MS and 1/2 MS medium to study the effect of different hormone concn.on the proliferation and rooting of P.cerasifera and establish the in vitro propagation system of P.cerasifera.[Result] The induction rate in the combination of 6-BA,NAA and NAA with proper concn.was higher than that in the treatment with single 6-BA.T5 with 6-BA and NAA at 1.0 and 0.2 mg/L resp.could get the induction of 75.0% and make axilla buds grow well.T6 with 6-BA and NAA at 2.0 and 0.2 mg/L resp.could get the proliferation coefficient of 7.67 and had buds of 4.76 whose height was over 2 cm.The optimum IBA concn,suiatable for rooting of P.cerasifera was 0.5 mg/L,with rooting of 98.3%.The survival rate for transplanting of tube seedlings was over 85%.[Conclusion] The best initial medium for in vitro culture of P.cerasifera was MS+6-BA1.0 mg/L+NAA 0.2 mg/L,the best medium for proliferation was MS+6-BA 2.0 mg/L+NAA+0.2 mg/L and the best medium for rooting was 1/2 MS+IBA 0.5 mg/L.
【Fund】: 重庆市教委重大平台建设项目(GCZX0713);; 重庆文理学院重点科研项目
【CateGory Index】: S687
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