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《Journal of Anhui Agricultural Sciences》 2008-21
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Identify and Characterize the PPARγ Ligand-binding Activity of Extracts of Cleistocalyx Operculatus

JIN Xiao-hui et al (South China Botanical Garden, Chinese Academy of Sciences, Guangzhou,Guangdong 510650)  
[Objective] To identify and characterize the peroxisome proliferator-activated receptor-γ(PPARγ) ligand-binding activity of 2,4-dihydroxy-6-methoxy-3,5-dimethylchalcone, a chalcone derivative extracted from the dried flower of Cleistocalyx operculatus.[Method] The PPARγ-transactivation activity of DMC was examined by using a GAL4 hybrid reporter gene assay. The effect of DMC on adipogenesis was measured by oil red O staining. The glucose uptake rate was measured by using [3H]-2-deoxy glucose in 3T3-L1 adipocytes after treatment with DMC. The effect of DMC on expression of PPARγ target genes in differentiated 3T3-L1 adipocytes was examined by real-time PCR. [Results] DMC can selectively activate PPARγ with a dose-dependent manner. Result of oil red O staining showed that DMC can promote adipocyte differentiation. DMC can also strongly enhance glucose uptake in 3T3-L1 adipocytes. Furthermore, the result of real-time PCR showed the DMC can modulate some PPARγ-responsive genes in differentiated 3T3-L1 adipocytes. [Conclusion] Our data establish DMC as a natural PPARγ ligand with capability of promoting glucose uptake in 3T3-L1 adipocytes.
【Fund】: 国家自然科学基金资助项目(30670457);; 国家973计划资助项目(2004CB720102)
【CateGory Index】: R284
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1 Ren Yanli et al.(College of Chemistry and Biological Science,Yili Normal University,Yining 835000,China);Study on Bacteriostasis Test of Xinjiang Wild Walnut Leave Extract[J];Anhui Agricultural Science Bulletin;2012-13
2 Bao Yumei et al.(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China);Survey and Evaluation of Chinese Medicine Resources in Malong Peak Cangshan Mountain[J];Anhui Agricultural Science Bulletin;2012-13
3 CHENG Hui-chang et al (Animal Medicine Department of Zhengzhou College of Animal Husbandry Engineering, Zhengzhou, Henan 450011);Experimental Comparison Study on the Effects of the Extraction from Chebule(Fructus chebulae) and Pomegranate Peel (Pericarpium granati) to the Movement of Isolated Intestine of Rabbit[J];Journal of Anhui Agricultural Sciences;2008-21
4 HONG Zu-can et al (Longyan Tobacco Industrial Co.ltd.,Longyan,Fujian 364021);Comparison on the Volatile Components in Different Years Old Morinda officinalis[J];Journal of Anhui Agricultural Sciences;2009-09
5 HU Yan-wu et al (Department of Pharmaceutics and Food Science,Tonghua Teachers College,Tonghua,Jilin 134002);Extraction and Content Determination of Polysaccharide in Cuscuta japonica Choisy in Changbai Mountain Area[J];Journal of Anhui Agricultural Sciences;2010-09
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7 GUO Hai-bin et al(College of Chemistry and Chemical Engineering,Nanjing University of Technology,Nanjing,Jiangsu 210009);Optimization of Microwave-assisted Extraction of the Total Flavonoids from Reed Leaf by Response Surface Methodology[J];Journal of Anhui Agricultural Sciences;2010-29
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10 XU Na NING Shu-xiang ZHANG Mei-yan(College of Life Science,Liaoning Normal University,Dalian Liaoning 116029);Study on Rapid Propagation of Chelidonium majus[J];Modern Agricultural Sciences and Technology;2010-21
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8 Deng Guang-hai;Li Hua;Gong You-ming;Pharmaceutical Department,Guangdong Provincial Hospital of TCM;;The Study of Determination the Content of Six Kinds of Alkaloids of Aconiti Kusnezoffii Radix and its Processed Products[A];[C];2014
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 LU Yan-hua 1, DU Chang-bin 1, WU Zi-bin 2, YE Chun-lin 1, LIU Jian-wen 1, WEI Dong-zhi 1 (1.State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, China; 2.Department of Biochemistry, The Chinese University of HongKong);Protective Effects of Cleistocalyx operculatus on Lipid Peroxidation and Trauma of Neuronal Cells[J];China Journal of Chinese Materia Medica;2003-10
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