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《Journal of Anhui Agricultural Sciences》 2008-21
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Identify and Characterize the PPARγ Ligand-binding Activity of Extracts of Cleistocalyx Operculatus

JIN Xiao-hui et al (South China Botanical Garden, Chinese Academy of Sciences, Guangzhou,Guangdong 510650)  
[Objective] To identify and characterize the peroxisome proliferator-activated receptor-γ(PPARγ) ligand-binding activity of 2,4-dihydroxy-6-methoxy-3,5-dimethylchalcone, a chalcone derivative extracted from the dried flower of Cleistocalyx operculatus.[Method] The PPARγ-transactivation activity of DMC was examined by using a GAL4 hybrid reporter gene assay. The effect of DMC on adipogenesis was measured by oil red O staining. The glucose uptake rate was measured by using [3H]-2-deoxy glucose in 3T3-L1 adipocytes after treatment with DMC. The effect of DMC on expression of PPARγ target genes in differentiated 3T3-L1 adipocytes was examined by real-time PCR. [Results] DMC can selectively activate PPARγ with a dose-dependent manner. Result of oil red O staining showed that DMC can promote adipocyte differentiation. DMC can also strongly enhance glucose uptake in 3T3-L1 adipocytes. Furthermore, the result of real-time PCR showed the DMC can modulate some PPARγ-responsive genes in differentiated 3T3-L1 adipocytes. [Conclusion] Our data establish DMC as a natural PPARγ ligand with capability of promoting glucose uptake in 3T3-L1 adipocytes.
【Fund】: 国家自然科学基金资助项目(30670457);; 国家973计划资助项目(2004CB720102)
【CateGory Index】: R284
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