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《Journal of Anhui Agricultural Sciences》 2008-22
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Determination of Quercetin in Yi Gan Qing Capsule by HPLC

WANG Min-zhi et al (College of Pharmacy,Southwest Jiaotong University,Emeishan,Sichuan 614202)  
[Objective ] The research aimed to establish the determination method of quercetin in Yi Gan Qing capsule by HPLC.[Method] HPLC was used for determination,the chromatographic conditions were as follows: Phenomenex Luna C18 column(4.6 mm×250 mm,5 μm),methanol-0.4% H3PO4(50∶50) as mobile phase,the detection wavelength of 360 nm,the flow rate of 1 ml/min and the column temperature of 30 ℃.[Result] Quercetin had good linear relationship with the peak area within the range of 0.054-1.08 μg,the correlation coefficient(r) was 0.999 88,the average recovery was 99.15% with RSD of 1.42%(n=6).[Conclusion] This determination method is simple,accurate and reliable,so it could be used as the determination method of quercetin content in Yi Gan Qing capsule.
【Fund】: 西南交通大学峨眉校区科技资助项目
【CateGory Index】: R286.0
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