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《Journal of Anhui Agricultural Sciences》 2008-23
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Preliminary Study on the Secondary Metabolites of Microbe under Oligotrophic Environment in Loess of Northern Shaanxi

LEI Chao et al(Ganquan Senior High School,Yan'an,Shaanxi 716100)  
[Objective] The study was to explore the suitable method to isolate and culture microbe for oligotrophic environment in loess plateau in northern shaanxi.[Method] With separation and selection method for oligotrophic medium and single hyphae apex taken method,the microbe under extreme oligotrophic environment in loess samples from northern Shaanxi were separated and purified.The secondary metabolites of the selected strains were analyzed through silicon thin-layer chromatography.[Result] Eighteen microbe strains under extreme oligotrophic environment were separated from loess plateau samples,and 2 microbe strains under extreme oligotrophic environment were separated from the weathering soil of stone samples.Among them,8 strains were separated from medium with pH of 4 and 12 strains were separated from medium with pH of 10.The secondary metabolites of JDA-03,JDA-04,JDA-05,JDA-06,JDA-07,JDB-11,JDB-13 strains were abundant,and there had terpene compounds in secondary metabolites of JDB-9 and JDB-10 stains.[Conclusion] This study could lay a foundation for development and utilization of the unique microbe in loess of northern Shaanxi.
【CateGory Index】: Q93
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