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《Journal of Anhui Agricultural Sciences》 2008-24
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Effects Chitosan Treatment on Drought Resistance of Alfalfa Seedlings

WANG Yan-feng et al(School of Life Science,Yan'an University,Yan'an,Shaanxi 716000)  
[Objective] The study aimed to preliminarily discuss the effect of chitosan treatment on the drought resistance of alfalfa seedlings under drought stress.[Method] The alfalfa seeds with uniform plumpness were cultured in incubator at 25 ℃.When 3-4 true leaves were gown out and put on the culture dishes they were sprayed by 0.1%,0.3%,0.5%,0.7% chitosan,with spraying the distilled water as CK,so as to study the effect of chitosan treatment with different concn.on the drought resistance of alfalfa seedlings.[Result] Under drought stress,compared with the control group,the proline content of alfalfa seedlings treated by chitosan with different concn.was increased slowly and its increasing in treatment group with 0.5% chitosan was slowest.POD activity of alfalfa seedlings in alfalfa seedlings treatments was raised obviously fast than that in the control group,with their increasing extent in different concn.treatments in order being 0.5% 0.7%0.3%0.1%,and POD activity of alfalfa seedlings in treatment groups was decreased slowly after reaching the peak value at the 9 th d under stopping water.[Conclusion] Treating alfalfa seedling by chitosan could regulate the ratio of proline accumulation,maintain and enhance the POD activity in alfalfa seedling under drought stress,thus increasing its drought resistance.The optimum concn.of chitosan for treating alfalfa seedlings was 0.5%.
【CateGory Index】: S541.9
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