Research on the Phagocytosis Condition of Chicken's Peripheral Blood Leucocytes
QIN Feng et al(Jiangsu R&D Center of Animal Pharmaceutical,Taizhou,Jiangsu 225300)
[Objective]The purpose was to ascertain the optimal conditions of phagocytosing Staphylococcus spp.by chicken's peripheral blood leucocytes.[Method]With Staphylococcus spp.10145 as tested materials,phagocytosis activity of chicken's peripheral blood leucocytes was detected by the method of Wright's staining.The effect of time,temperature and concentration of Staphylococcus spp.10145 on phagocytosis percentages(PP) and phagocytosis index(PI) were optimized and their morphologic characteristics were observed by microscope.[Result]The results indicated that the concentration of Staphylococcus spp.10145 was the primary factor of effecting leucocytes phagocytosis index.The optimal phagocytosis conditions were at the temperature of 41℃,time of 40 min and concentration of Staphylococcus spp.10145 of 5×106/ml.The effect degree of various factors on leucocytes phagocytosis index was the concentration of Staphylococcus spp.10145 time temperature.[Conclusion] This study will provide a more simple and reliable method for detecting leucocytes phagocytosis function.