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《Journal of Anhui Agricultural Sciences》 2008-24
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Determination of Swertisin Content in Gentiana straminea Maxim. by RP-HPLC

LI Mei-lan(College of Chemistry,Qinhai University,Xining,Qinghai 810007)  
[Objective] The aim of this paper was to establish a method for the determination of swertiamarin content in Gentiana straminea Maxim.by RP-HPLC.[Method] The samples were separated in the column of Kromasil C18(250 mm×4.6 mm,5 μm).Then,it was eluted with 26% methanol / water(0.04% H3PO4) with 240 nm detected wavelength and 1 ml/min flow rate under 30 ℃column temperature.[Result] Swertiamarin was base-isolated.The linear range of swertiamarin was between 0.009 5 and 2.900 0 μg(r=0.999 9).The addition recovery rate was 99.5%(RSD=3.2%).[Conclusion] The method was rapid,precise and credible.
【Fund】: 青海省重点科技攻关项目(2006-N-141)
【CateGory Index】: R284
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Chinese Journal Full-text Database 2 Hits
1 LIN Peng-cheng WANG Yong-ju ZHU Cun-en JI Shou-xiang LIANG Yong-xin LU Yong-chang (Department of Chemistry,Qinghai National Institutes,Xining 810007,China);Simultaneous Determination and Evaluation of Two Irdoids in Gentiana straminea from Different Areas in Qinghai Province[J];Chinese Pharmaceutical Journal;2006-10
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2 WU Jiang et al (The Department of Science and Technology of Qinhai Nationlities University,Xining,Qinhai 810007);Determination of Content Ursolic Acid and Oleanolic Acid of Tebitan Herbal Medicine Gentianaceae by RP-HPLC[J];Journal of Anhui Agricultural Sciences;2006-11
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5 JIA Hong-guang(Qinghai University,Xining,Qinghai 810016);Determination of Swertisin and Swertianolin Contents in Gentianopsis paludosa(Munro)Ma by RP-HPLC[J];Journal of Anhui Agricultural Sciences;2008-24
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7 TIAN Chun-yuan(School of Life Science and Technology,Xiaogan University,Xiaogan,Hubei 432000);Preliminary Study on Pharmacognosy of Chickpea[J];Journal of Anhui Agricultural Sciences;2009-18
8 ZHANG Cheng-wei1,ZHOU Ya-qiu2,CHEN Lei1 (1.Anhui College of Traditional Chinese Medicine;2.Anhui Huakang Medicine Technology Development Co.Ltd.,Hefei,Anhui 230038);Advances of the hyperin pharmacology research[J];Anhui Medical and Pharmaceutical Journal;2007-11
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