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《Journal of Anhui Agricultural Sciences》 2008-24
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Determination of Swertisin Content in Gentiana straminea Maxim. by RP-HPLC

LI Mei-lan(College of Chemistry,Qinhai University,Xining,Qinghai 810007)  
[Objective] The aim of this paper was to establish a method for the determination of swertiamarin content in Gentiana straminea Maxim.by RP-HPLC.[Method] The samples were separated in the column of Kromasil C18(250 mm×4.6 mm,5 μm).Then,it was eluted with 26% methanol / water(0.04% H3PO4) with 240 nm detected wavelength and 1 ml/min flow rate under 30 ℃column temperature.[Result] Swertiamarin was base-isolated.The linear range of swertiamarin was between 0.009 5 and 2.900 0 μg(r=0.999 9).The addition recovery rate was 99.5%(RSD=3.2%).[Conclusion] The method was rapid,precise and credible.
【Fund】: 青海省重点科技攻关项目(2006-N-141)
【CateGory Index】: R284
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 LIN Peng-cheng WANG Yong-ju ZHU Cun-en JI Shou-xiang LIANG Yong-xin LU Yong-chang (Department of Chemistry,Qinghai National Institutes,Xining 810007,China);Simultaneous Determination and Evaluation of Two Irdoids in Gentiana straminea from Different Areas in Qinghai Province[J];Chinese Pharmaceutical Journal;2006-10
2 Lin Pengcheng 1, Ye Zhaohui 1, Lu Yongchan 1, Zhao Suqin 1, Hu Fengzu 2, Ji Lanju 2 (1.Qinghai Nationalities Institutes, Xining 810007; 2.Northwest Planteau Institute of Biology, The Chinese Academy of Sciences, Xining 810001);Content Determination of Loganic Acid and Gentiopicroside in Tebitan Herbal Medicines Gentiana macrophylla and G.straminea by HPLC[J];Journal of Chinese Medicinal Materials;2004-11
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIANG Yong-xin et al(Chemistry Department of Qinghai Nationalities Institutes,Xining,Qinghai 810007);Simultaneously Determination of Four Effective Components in Swertia tetraptera Maxim.by HPLC[J];Journal of Anhui Agricultural Sciences;2006-10
2 WU Jiang et al (The Department of Science and Technology of Qinhai Nationlities University,Xining,Qinhai 810007);Determination of Content Ursolic Acid and Oleanolic Acid of Tebitan Herbal Medicine Gentianaceae by RP-HPLC[J];Journal of Anhui Agricultural Sciences;2006-11
3 JI Shou-xiang(Pharmacy Department of Qinghai Nationalities Institutes,Xining,Qinghai 810007);Simultaneously Determination of Three Efective Components in Swertia przewalskii with HPLC[J];Journal of Anhui Agricultural Sciences;2007-09
4 LIU Chao-xiang et al(Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province,Xuzhou,Jiangsu 221116);Determination on the Chemical Constituents and the Biological Activity of Volatile Oil from Leaves of Michelia maudiae Dunn[J];Journal of Anhui Agricultural Sciences;2008-17
5 JIA Hong-guang(Qinghai University,Xining,Qinghai 810016);Determination of Swertisin and Swertianolin Contents in Gentianopsis paludosa(Munro)Ma by RP-HPLC[J];Journal of Anhui Agricultural Sciences;2008-24
6 HUO Qing (Biological Medicine Department,Biochemical Engineering Institute of Beijing Associated University,Beijing 100023);Study on Extraction of Effective Components of Ailanthus altissima Swingle with Isolation Technology of Thin-layer Chromatography[J];Journal of Anhui Agricultural Sciences;2008-25
7 TIAN Chun-yuan(School of Life Science and Technology,Xiaogan University,Xiaogan,Hubei 432000);Preliminary Study on Pharmacognosy of Chickpea[J];Journal of Anhui Agricultural Sciences;2009-18
8 ZHANG Cheng-wei1,ZHOU Ya-qiu2,CHEN Lei1 (1.Anhui College of Traditional Chinese Medicine;2.Anhui Huakang Medicine Technology Development Co.Ltd.,Hefei,Anhui 230038);Advances of the hyperin pharmacology research[J];Anhui Medical and Pharmaceutical Journal;2007-11
9 HU Hao-bin,ZHENG Xu-dong,HU Huai-sheng,ZHANG Yu-quan(Dept.Chem.,Longdong Univ.,Qingyang 745000,Gansu,China);Studies on the chemical constituents of the volatile oil from leaves and stems of Origanum vulgare L.[J];Journal of Baoji College of Arts and Science(Natural Science Edition);2005-04
10 GONG Chuan-zhi1,DING Bin2(1.Northeast Branch Company,China Petroleum Engineering Co.,Ltd.,Jilin 132002,China;2.Jilin Institute of Chemical Technology,Jilin 132022,China);Preparation of diethyl succinate by catalytic esterification and adsorption dehydration[J];China Surfactant Detergent & Cosmetics;2008-04
China Proceedings of conference Full-text Database 3 Hits
1 ZONG Yu-ying1 , IP Siu-po1, DONG Ting-xia2 , CHE Chun-tao1*(1. School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China; 2. Department of Biology and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, China);Dertermination of morusin in Cortex Mori[A];[C];2006
2 Lai Xiao-ping, Chen Jian-nan, Ye Mu-rong, Hou Shao-zhen, Li Geng- (Guangzhou University of Traditional Chinese Medicine, Guangzhou, 510405 );The therapeutic Effect of Isodon lophanthoides var.gerardianus (Benth.) H.Hara on liver-injured animal models[A];[C];2006
3 YANG Zai-bo~(*1) ZHAO Chao~2 MAO Hai-li~1 LONG Cheng-mei~1 ZHONG Cai-ning~1 SUN Cheng-bin~1 (1.Department of Chemistry;Qiannan Normal University for Nationalities;Duyun;Guizhou,558000; 2.Research Center of the Quality Control of Natural medicines of Guizhou Normal University,Guiyang 550001);Analysis compares the root and the stem-leaf the Volatile oil from Peucedanupraerutorum Dunn in Guizhou[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 HU Fengzu, SONG Yali, LIU Mei, SHI Zhixian(Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, Xining 810001, China);Analysis of Medicinal Bioactive Composition of Gentianaceae in Qinghai-Tibet Plateau by High Performance Liquid Chromatography[J];Chinese Journal of Chromatography;2003-01
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