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《Journal of Anhui Agricultural Sciences》 2008-25
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Determination of Hypericin in Yiganqing Capsule by HPLC

YUAN Lin et al (School of Pharmacy,Southwest Jiaotong University,Emei,Sichuan 614202)  
[Objective] The research aimed to establish HPLC method for determination of hypericin in Yiganqing capsule.[Method] Two different methods of ultrasonic extraction and heating reflux extraction were applied to extract hypericin from the capsule's content in different solvents.The extraction effects were evaluated through the determination of hypericin by HPLC to find out the optimum extraction condition.HPLC conditions were as follows: Phenomenex Luna C18(4.6 mm×250 mm,5 μm) as analytic column, methanol-2.5 g/L KH2PO4(95∶5,V/V) as mobile phase,the detection wavelength was set at 588 nm,and the flow rate was 1.0 ml/min.[Result] The optimum extraction method was that,the Yiganqing capsule's content was extracted with methanol for 30 minutes by heating reflux at 90 ℃ in a water bath.The linearity of hypericin was good at the range of 0.108-1.080 μg,r=0.999 84,and the mean value of recovery was 98.8% with RSD of 1.65%.[Conclusion] HPLC method was established for determination of hypericin in Yiganqing capsule with good stability and accuracy.It could be used for the quality control of Yiganqing capsule.
【CateGory Index】: R286
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