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《Journal of Anhui Agricultural Sciences》 2008-25
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Studies on Enhancement of Tissue-culture Efficiency in Mature Embryo of Parental Lines of Indica Hybrid Rice

SHEN Xian-hua et al (Rice Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang,Jiangxi 330200)  
Tissue culture efficiency of rice was highly dependent on the genotypes.It was of great paramount to study and set up an efficient tissue culture system focused on the particular genotypes of rice.The research screened more suitable callus induction medium,compared the effectiveness of 6-BA and Kt in the plant regeneration,and optimized the combinations and concentrations of plant growth hormones in plant regeneration medium.The results showed that the average CIR and ECIR obtained on MS medium were 83.47% and 38.59%,respectively,being 3.4% and 7.42% higher than on CC medium,and among the 16 tested genotypes, 12 had simultaneously higher CIR and ECIR on MS than on CC.In addition,earlier response and more rapid growth of calli with lower rate of necrosis were observed on MS.Therefore,it indicated that MS medium had a broader spectrum in terms of callus induction from mature embryos of the indica hybrid rice parental lines.Comparison of the effectiveness of 6-BA and Kt showed that all of the 6 tested restorer lines displayed a higher GPRR on the medium consisting of 6-BA than that of Kt,and on average,the GPRR was 10.65% higher compared to the use of Kt.Hence,it was preliminarily concluded that 6-BA had a better effectiveness than Kt for plant regeneration in indica hybrid rice.The orthogonal test showed that the optimal combination and concentration of hormones for 2 genotypes,such as R998 and R463,were 3 mg/L 6-BA+0.5 mg/L NAA+1 mg/L Kt,while the optimal combination and concentration of hormones for R253 and R402 were 1 mg/L 6-BA+1 mg/L NAA and 2 mg/L 6-BA+0.5 mg/L Kt,respectively.In general,the orthogonal test with the hormones obtained very significant results in increasing the green plant regeneration rates of rice genotypes.Therefore,it was indicated that optimization of combinations and concentrations of hormones could further increase the GPRR of indica genotypes,especially,of those recalcitrant genotypes of indica rice.
【Fund】: 江西省学科带头人培养项目;; 江西省农业科学院创新基金项目
【CateGory Index】: S511.21
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【References】
Chinese Journal Full-text Database 1 Hits
1 LV Meng-yu,WANG Hai-bo,GAO Yi-ping(Institute of Genetic and Physiology of Hebei Academy of Agriculture and Forestry Sciences,Hebei Center of Plant Genetic Transformation,Shijiazhuang 050051,China);Effects of Inoculation Methods of Rice Mature Embryo on Callus Induction[J];Journal of Hebei Agricultural Sciences;2009-05
【Citations】
Chinese Journal Full-text Database 8 Hits
1 He Qiongying Wang Runhua(Department of Agronomy);EFFECTS OF SORBITOL ON INDICA RICE CALLUS CULTURE[J];Journal of South China Agricultural University;1991-01
2 XIAO Xiang-wen1,LI Ping1, YANG Zheng-lin1, ZHONG Bing-qiang1, HOU Lei2, LI De-mou2, HE Guang-hua 1* (1. Rice Institute of Southwest Agricultural University, Chongqing 400716, China; 2. Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Southwest Agricultural University, Chongqing 400716, China);A COMPARATIVE STUDY OF THE REGENERATION CAPACITY OF 5 GOOD FERTILITY-RESTORING LINES OF INDICA RICE[J];Journal of Southwest Agricultural University;2005-03
3 Tian Wenzhong(Institute of Genetics Academia Sinica Beijing 100101)Iann Rance;Elunialai Sivamani;Claude Fauquet;Roger N.Beachy(International Laboratory for Tropical Agricultural Biotechnology The Scripps Research Institute La Jolla CA 92037 USA);Improvement Of Plant Regeneration Frequency in vitro In Indica Rice[J];ACTA GENETICA SINICA;1994-03
4 SUN Zongxiu, SI Huamin, CHENG Shihua, ZHAN Xiaoyan(China National Rice Research Institute. Hangzhou 310006);Effect of Maltose on Efficiency of Anther Culture of Rice[J];Chinese Journal of Rice Science;1993-04
5 LUO Qiong; HU Yanyu; ZHOU Kaida (Rice Research Institute, Sichuan Agricultural University, Wengjiang 611130);Role of Endogenous Hormones in Tissue Culture of Mature Rice Embryo[J];CHINESE JOURNAL OF RICE SCIENCE;1998-04
6 ZHANG Ping 1, ZUO Shi-min 1 , LI Ai-hong 1,2 , ZHANG Ya-fang 1 , CHEN Zong-xiang 1, PAN Xue-biao 1,*(1College of Agriculture, Yangzhou University, Yangzhou 225009, China; 2Agricultural Science Institute of Lixiahe Area of Jiangsu Province, Yangzhou 225002, China);Factors Affecting Rice Transformation Frequency Mediated by Agrobacterium[J];Chinese Journal of Rice Science;2004-01
7 Wang Xingchun Feng Hongming Xu Huilan Yang Changdeng (Key Laboratory for Rice biology, Ministry of Agriculture, Hangzhou,310006);Study on Raising the Culture Ability of Mature Embryos in High quality Indica Rice[J];Seed;2001-01
8 LIU Qiao-Quan; ZHANG Jing-Liu; WANG Zong-Yang; HONG Meng-Min and GU Min-Hong( Department of Agronomy Agricultural College ,Yangzhou 225009 China;Shanghai Institute of Plant Physiology, Chinese Academy of Sciences,Shanghai 200032, );A Highly Efficient Transformation System Mediated by Agrobacterium tumefaciens in Rice(Oryza sativa L.)[J];ACTA PHOTOPHYSIOLOGICA SINICA;1998-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Jinyuan, Shi Xiangyu, Chen Xiao, Zeng Zhaohai,Zhou Zhuhua & Chen Jianai(Institute of Atomic Energy Application in Agriculture,Shandong Acodemy of Agricultural Sciences,Jinan 250100);Study on Transformation of Wheat and Maize Via Gene-gun Bombardment[J];SHANDONG AGRICULTURAL SCIENCES;1999-02
2 LIU Xiang-ling~(1,2),WANG Yu-zhen~1,LUO Jing-lan~1(1.Center for Agricultural Resources Research,Institute of Genetic and Developmental Biology,Chinese Academy of Science,Shijazhuang 050021,China;2.Graduate University of Chinese Academy of science,Beijing 100039,China);Study on callus induction and regeneration from rice mature embryo scutum[J];Shandong Agricultural Sciences;2005-05
3 Lin Yi ( Department of Science and technology management,Suzhou Agricultural Science Research Institute,Suzhou 23400,China);Preliminarily Study On Rice Callus Propagation in Liquid Culture[J];Anhui Agricultural Science Bulletin;2010-11
4 WANG Xiu-qin et al(College of Agronomy,Shandong Agricultural University,Taian,Shandong 271018);Research Progress in Transformation Maize Mediated with Agrobacterium Tumefaciens[J];Journal of Anhui Agricultural Sciences;2006-18
5 JIANG Gang-qiang et al (Technical Center,Xinjiang Entry-exit Inspection and Quarantine Bureau,Urumqi,Xinjiang 830063);Study on the Callus Induction,Subculture and Plant Regeneration of Chenopodium glaucum Linn[J];Journal of Anhui Agricultural Sciences;2009-29
6 WANG Lan et al (Key Laboratory of Plant Molecular Breeding of Guangdong Province,College of Agriculture,South China Agricultural University,Guangzhou,Guangdong 510642);Progress in Genetic Transformation Mediated by Agrobacterium tumefacien in Rice[J];Journal of Anhui Agricultural Sciences;2009-30
7 SHI Li-li et al (Department of Agronomy,Tianjin Agricultural University,Tianjin 300384);Comparison of the Regeneration Capacity of 5 Japonica Rice Varieties[J];Journal of Anhui Agricultural Sciences;2009-31
8 ZENG Sheng-jian et al(College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009);Callus Induction and Plant Regeneration from Mature Seeds of Perennial Ryegrass[J];Journal of Anhui Agricultural Sciences;2009-35
9 WANG Wei-xu et al(Key Laboratory of State Ethnic Affairs Commission for Biological Technology,College of Life Science,South-Central University for Nationalities,Wuhan,Hubei 430074);Study on the Factors of Genetic Transformation for Indica Rice Kasalath[J];Journal of Anhui Agricultural Sciences;2010-04
10 DONG Kuan-hu,YU Na,CHENG Yu-hong (College of Animal Science and technology,Shanxi Agricultural University,Taigu,Shanxi Province 030801,China);Callus Induction and Plant Regeneration from Euphyllas of Bothriochloa ischaemum[J];Acta Agrestia Sinica;2009-02
China Proceedings of conference Full-text Database 4 Hits
1 Liu Yanzhi;Li Junbo;Wang Yumin;Wang Zhongwei;Ji Feng;Dong Yingshan Jilin Province Key Open Lab. On Agro-biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling 136100;Establishment of high efficiency donee system in rice genetic transformation[A];[C];2003
2 Zhang Hongyu;Wang Xudong;Wang Xiuhong;Wu Xianjun;Luo Qiong Rice Reasearch Institute, Sichuan Agricultural University, Wenjiang, 611130;Studies on the Influence of Endogenous Hormone on Rice Anther Culture[A];[C];2003
3 ZHUO,Chunliu,CAI Jiongliang,FANG Junyang,ZHOU Ming,GUO Zhenfei (Biotechnology Laboratory for Turfgrass and Forages,Department of Plant Science,College of Life Sciences,South China Agricultural University,Guangzhou 510642:China.);Early light induced protein(ELIP) gene from Medicago falcata confers resistance to abiotic stresses[A];[C];2010
4 Qin Hongtao Xiao Han Sun Zongxiu Xu Tong( 1 Department of Plant Protect, Zhejiang University, I langzhou 310029 ) ( 2 Rice Biology Lxab of Agriculture Department, Rice Research Institute of China, I langzhou 310006 );Enhance Disease Resistance of Transgenic Rice Plants with an Endochitinase Gene ThEn - 42 from Trichoderma harzianum[A];[C];2000
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Fang~1,Lü Wei-tao~1,CUI De-cai~2,ZHAO Tan-fang~1(1.College of Agronomy,Shandong Agricultural University,Taian 271018,China;2.College of Life Sciences,Shandong Agricultural University,Taian 271018,China);Obtained transgenic wheat induced by anti-PLDγ gene and its primary identification of powdery mildew resistance[J];Shandong Agricultural Sciences;2006-02
2 ZHENG Wen-jing et al (Rice Institute of Liaoning Province,Shenyang,Liaoning 110161);Study on the Difference of Tissue Culture with Different Explants in Rice and Their Offspring Variations[J];Journal of Anhui Agricultural Sciences;2008-04
3 Lei Bojun Yin Guangchu Lu Caihua Qian Hua Zhang Kaiwang Zhou Sijun Wang Shulin(Soybean Research Institute HAAS Harbin China);STUDY OF EXOGENOUS DNA DIRECTLY TRANSFERED INTO SOYBEAN[J];Soybean Science;1991-01
4 Liu Zhaojun (Biotechnology Research Centre, Heilongjiang Academy of Agricultural Science, Harbin 150086);THE TECHNIQUE ON EXOGENOUS DNA INTRODUCED DIRECTLY INTO SOYBEAN AND ITS APPLICATION IN SOYBEAN BREEDING[J];Soybean Science;2004-04
5 FAN Qin,XU Xin ping,HUANG Xiao le,LI Bao jian *(Life Science Research Center of Zhongshan University,Guangzhou 510275,China);Callus formation and plant regeneration of indica rice variety Pei'ai 64S[J];Acta Botanica Boreali-occidentalia Sinica;2002-06
6 WANG Yan-jie,SHEN Jia-heng~*(Department of Biology,College of Life and Environment Science,Harbin Normal University,Harbin 150080,China);Probing into Cytological Embryology Mechanism of Pollen-tube Pathway Transgenic Technology[J];Acta Botanica Boreali-Occidentalia Sinica;2006-03
7 YIN Zhong-ming,ZHOU Yong-guo,XU Shen-zhong,LI Jian-yue(College of Life and Environment Science,Shanghai Normal University,Shanghai 200234,China);Reduction of Amylose Contents in Transgenic Rice with Different Copies of Anti-Waxy Gene[J];Acta Botanica Boreali-Occidentalia Sinica;2007-10
8 LIU Li-xia,HU Xiao-li,ZONG Xiao-juan,LIU Yu-kun,LI De-quan(State Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Tan’an,Shandong 271018,China);Maize Genetic Transformation Mediated by Agrobacterium tumefaciens and Optimization of the Differentiation and Rhizogenesis Conditions[J];Acta Botanica Boreali-Occidentalia Sinica;2007-11
9 Liu Zhixue, Zhang Xu , Xu Ya’nan, He Yiyuan, Ma Xiangqian, Shen Daleng, Tang Kexuan (Institute of Genetics, Department of Microbiology and Microbial Biotechnology );Optimization of the Conditions for Rice Genetic Transformation Mediated by Agrobacterium Tumefaciens LBA4404/pCAMBIA Series[J];JOURNAL OF FUDAN UNIVERSITY;1999-04
10 LIU Zhi xue, MA Xiang qian, HE Yi yuan,Xu Ya nan, YE Ming ming, TANG Ke xuan (Institute of Genetics);An Improved Assisting Method for Genetic Trans formation via Agrobacterium tumefaciens[J];JOURNAL OF FUDAN UNIVERSITY;1999-05
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 WANG Fang1,WANG Lei1,ZHANG Jun1,YIN Sheng-wu1,HUA Ze-tian1,2(1.College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China; 2.Tianjin Tianlong Agricultural Science and Technology Co.,Ltd.,Tianjin 300457,China);Optimization of Tissue Culture Condition of Mature Embryo of Rice Liaojing 294 with High Plumule Ratio[J];Hubei Agricultural Sciences;2012-05
2 ZHOU Xiao-hong1,ZHOU Yan-wei1,ZHANG Yuan-li1,SHI Ji-sen1,ZHENG Ren-hua2,CHEN Jin-hui1(1.Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education,College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;2.Fujian Academy of Forestry,Southern Mountain Timber Forest Cultivation Lab,State Forestry Administration,Fuzhou 350012,Fujian,China);Studies on Adventitious Bud Proliferation of Chinese Fir[J];Forest Research;2013-03
3 ZHOU Jie,WANG Chun-tai,XU Xin(Key Laboratary of State Ethnic Affaire Commission for Biological Technology,College of Life Science,South-Central University for Nationalities,Wuhan 430074,China);Effect of Pretreatment on Mature Embryo Callus Indunction of Indica Rice YTB[J];Seed;2011-11
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Chen Yunzhao Wang Yugao (Shanxi Agricultural University);THE EFFECT OF VARIOUS CONCENTRATIONS AND RATIO OF KINETIN AND NAA ON ORGAN DIFFERENTIATION OF TISSUE CULTURE OF SOYBEAN[J];Soybean Science;1984-04
2 Piao Zhongze(Crop Research Institute, Tianjin Academy of Agricultural Sciences, Tianjin);A Study on Dynamic Changes of Uncleic Acid, Protein and Zyme of Rice Callus in Continuous Culture[J];Acta Agriculturae Boreali—Sinica;1990-04
3 YUAN Ling (Department of Biology,Xiaogan University,Xiaogan 432100,China);Role of copper or silver on redifferentiation frequency of rice callus[J];Hubei Agricultural Sciences;2003-01
4 He Qiongying Wang Runhua(Department of Agronomy);EFFECTS OF SORBITOL ON INDICA RICE CALLUS CULTURE[J];Journal of South China Agricultural University;1991-01
5 Mei Chuansheng, Zhang Jinyu and Wu Guangnan (Institut of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences);Improving Regeneration Rate of Anther Culture in Indica Rice (Oryza sativa L. subsp. indica)[J];Jiangsu Journal of Agricultural Sciences;1988-02
6 Mi Qingli He Sijie Cao Shouyun Li Liangcai Tian Wenzhong (Institute of Genetics, Academia Sinica, Beijing 100101);Differences in Response to Biolistic Transformation between Indica and Japonica Rice and the Way to Increase Their Efficiency[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1998-03
7 Luo Qiong et al(Rice Research Institute, SiehuanAgricultural University/Yaan, Sichuan, China 625014);STUDIES ON INCREASING RICE ANTHER CULTURE EFFECTIVENESS[J];Journal of Sichuan Agricultural University;1995-04
8 Xiang Youbin 1 Liang Zhuqing 1 Gao Mingwei 1 Shu Qingyao 1 Ye Gongyin 2 Cheng Xiongying 1,3 I.Altosaar 3 1(Institute of Nuclear Agricultural Sciences,Zhejiang Agricultural University,Hangzhou 310029) 2(Department of plant pr;Agrobacterium mediated Transformation of Insecticidal Bacillus thuringiensis cryIA(b) and cryIA(c) Genes and Their Expression in Rice[J];CHINESE JOURNAL OF BIOTECHNOLOGY;1999-04
9 TSAI I-SHUN,ZHANG BAO-HENG AND SUN WEI (Institute of Genetics,Fudan University) SHAO YOU AND WANG DE-LT (Department of Agronomy,Shanghai Agricultural College);CALLUS INDUCTION AND PLANT REGENERATION IN DEHULLED RICE SEEDS OF THE HYBRID RICE IN ORYZA SATIVA SUBSP.JAPONICA[J];Acta Biologiae Experimentalis Sinica;1982-03
10 Peng Yanhua, Liu Chengyun;RECENT ADVANCES IN RELATION BETWEEN ABA AND EMBRYOGENESIS AND THE MODE OF ABA ACTION[J];Journal of Wuhan Botanical Research;1991-03
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